A topical composition
A topical composition, the technology of the composition, applied in the field of topical compositions for skin lightening, can solve problems such as difficulty
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[0119] Material:
[0120] - Green tea (Synthite catalog number SGT1006),
[0121] -Black tea prepared using the traditional black tea process using the leaves of the tea tree,
[0122] - Glacial acetic acid (Merck-45754),
[0123] - polystyrene divinylbenzene resin (Diaion HP20 Supelco 13607),
[0124] - Tanninase (Kikkoman KT50)
[0125] - primary (primary) neonatal epidermal human melanocytes (Lonza catalog number CC-2504),
[0126] Media (Cascade Biologics catalog number MEPI500CA),
[0127] - Dulbecco's Modified Eagle Medium (DMEM; Gibco catalog number 31600034),
[0128] - MBM-4 Melanocyte Growth Basal Medium (Lonza Cat# CC-3250); and
[0129] - Alpha-Melanocyte Stimulating Hormone (α-MSH; R&D System-3013).
[0130] Preparation of DGTE
[0131] Step a): The tea material is subjected to enzymatic hydrolysis.
[0132] Prepare a 10% black tea solution in deionized water. The solution is heated to 70°C, if necessary, to dissolve the black tea in the water. 10...
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