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Aldehyde synanthrin and preparation method and application thereof

A technology of aldehydlated inulin and ylated inulin, which is applied in the direction of pharmaceutical formulations, medical preparations with no active ingredients, medical preparations containing active ingredients, etc., can solve the problem that clinical islet transplantation cannot be widely promoted and applied, The expected success rate is only 50%, and achieve the effects of good local vascularization, guaranteed survival rate and activity, and mild reaction conditions

Active Publication Date: 2021-04-16
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the transplantation strategy has been able to achieve certain results with the improvement of islet isolation technology and the improvement of immunosuppressive treatment for more than ten years, clinical islet transplantation is still not widely promoted and applied, and the expected success rate is only 50%.

Method used

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  • Aldehyde synanthrin and preparation method and application thereof
  • Aldehyde synanthrin and preparation method and application thereof
  • Aldehyde synanthrin and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0037] This embodiment is a preparation method of aldylated inulin, the preparation method comprising the following steps:

[0038] dissolving inulin with a molecular weight of 5000 in deionized water to obtain an inulin solution;

[0039] Adding an aqueous solution of sodium periodate to the inulin solution and stirring and reacting for 45 hours under the condition of avoiding light and room temperature to obtain a reaction solution, wherein the molar ratio of the structural unit of inulin to sodium periodate is 5:1;

[0040] The reaction solution was dialyzed with deionized water for 40 hours and freeze-dried to obtain the above-mentioned aldehylated inulin.

Embodiment 2

[0042] This embodiment is a preparation method of islets coated with aldylated inulin, the preparation method comprising the following steps:

[0043] Dissolving the aldehylated inulin in sterile PBS buffer solution to obtain an aldehylated inulin solution with a mass concentration of 1 mg / mL;

[0044] Add islets to the aldehylated inulin solution, and then add four-arm amino PEG, and react for 2 hours at room temperature under stirring conditions to obtain the above-mentioned aldehylated inulin-coated islets, wherein every 5 mg of aldylated inulin corresponds The amount of islets added was 100; the molar ratio of aldylated inulin to four-arm amino PEG was 10:1.

Embodiment 3

[0046] This embodiment is a preparation method of islets coated with aldylated inulin, the preparation method comprising the following steps:

[0047] Dissolving the aldehylated inulin in sterile PBS buffer solution to obtain an aldehylated inulin solution with a mass concentration of 5 mg / mL;

[0048] Add islets to the aldehylated inulin solution, and then add four-arm amino PEG, and react for 2 hours at room temperature under stirring conditions to obtain the above-mentioned aldehylated inulin-coated islets, wherein every 5 mg of aldylated inulin corresponds The amount of islets added was 20; the molar ratio of aldylated inulin to four-arm amino PEG was 10:1.

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Abstract

The invention discloses aldehyde synanthrin and a preparation method and application thereof. The preparation method comprises the following steps of: dissolving synanthrin in deionized water, and obtaining a synanthrin solution; adding a sodium periodate aqueous solution into the synanthrin solution, and carrying out a stirring reaction for 24-72 h in a dark place at a room temperature to obtain a reaction solution; and dialyzing the reaction solution, and freeze-drying to obtain the aldehyde synanthrin. The aldehyde synanthrin prepared by adopting the method can form a coating on the surface of pancreas islet or cell mass, can avoid attack of an in-vivo immune system, is stable in overall structure of pancreas islet or cell mass, high in cell survival rate and intact in form, can release insulin under high glucose stimulation, is good in local vascularization, and facilitates the culture of the in-vitro pancreas islet and the transplantation of the in-vivo pancreas islet.

Description

technical field [0001] The invention relates to the technical field of cell encapsulation materials, in particular to an aldylated inulin and its preparation method and application. Background technique [0002] Diabetes is the third largest chronic disease in the world, second only to cardiovascular disease and cancer in terms of morbidity and mortality. Diabetes itself is also one of the main pathogenic factors leading to cardiovascular disease and cancer. Current therapeutic strategies for diabetes mainly rely on exogenous insulin supplementation. However, although insulin replacement therapy has made great progress, exogenous insulin supplementation cannot accurately control blood glucose levels due to complex factors such as changes in the patient's own metabolism or further development of the disease. Insulin injections can also cause systemic blood sugar fluctuations, with the risk of severe hypoglycemia. Moreover, supplementing exogenous insulin cannot fundamental...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K47/36A61K35/39A61K35/12A61K35/44A61K35/14A61P3/10
Inventor 李宸张闯年张明明周志敏
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI