Goldfish gene editing technology and method for creating new goldfish variety by using goldfish gene editing technology

A technology of gene editing and new species, applied in the field of fish molecular design and breeding, can solve the problems of lack of fast and efficient systematic methods, achieve the effects of reducing background differences, avoiding off-target phenomena, and improving gene editing efficiency
CN112680447APending Publication Date: 2021-04-20INST OF AQUATIC LIFE ACAD SINICA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF AQUATIC LIFE ACAD SINICA
Publication Date
2021-04-20

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Abstract

The invention relates to a fish molecular design breeding technology, in particular to a goldfish gene editing technology and a method for creating a new goldfish variety by using the gene editing technology. The method comprises steps of knockout target design, microinjection, fertilization incubation, gynogenesis and high-temperature sex reversal. According to the invention, a CRISPR / Cas9 gene editing technology is established on goldfish, so that a target phenotype is generated on F0 generation goldfish, an efficient gynogenesis condition of pleated Thai lion goldfish is determined through experiments, homozygotes are obtained in F1 generation, and a large number of groups are expanded in F2 generation through temperature-induced reversion. Through a molecular breeding means, transfer of a single target character is rapidly achieved, the goldfish gene editing technology and the method can be used for creating a new goldfish variety, provides technical support for molecular design and breeding of goldfish, and is beneficial to promote development of goldfish molecular breeding technology.
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Description

technical field

[0001] The invention relates to fish molecular design and breeding technology, in particular to a goldfish gene editing technology and a method for creating new goldfish varieties using the gene editing technology.

[0002] technical background

[0003] Goldfish originated from the tetraploid crucian carp in my country. It is an ornamental fish formed after hundreds of years of artificial selection, and it can be called the quintessence of our country. In the history of goldfish development, due to mutations, goldfish body color, body shape and almost all external organs have undergone obvious changes. Due to artificial selection, these mutations have been retained, and these mutations have been hybridized and recombined to form multiple compound variant goldfish; Today, there are more than 300 species of goldfish.

[0004] Traditional goldfish are kept in fish pots, and the breeding of species is mainly based on looking down. The popularity of fish tanks ha...

Claims

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