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Tissue culture method for euryodendron excelsum

A tissue culture, porcine blood wood technology, applied in the field of plant biology, can solve the problems of limited number of circle branches, difficult to obtain a large number of seedlings, often underdeveloped embryos, etc., and achieves good market prospects, less investment, and high genetic stability. Effect

Active Publication Date: 2021-04-23
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bloodwood can be propagated by sowing, but its natural seed setting rate is low, and the embryos are often underdeveloped, resulting in low germination rate
The number of ring branch propagation of pig blood wood is also very limited, so it is difficult to obtain a large number of seedlings through conventional propagation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Disinfection method of explant: take the blue-black fruit on the vigorous growth plant of Euryodendron excelsum H.T.Chang, remove the pulp after soaking in water, obtain well-developed seeds, and first soak in 75% ethanol aqueous solution by volume fraction 10 seconds, and then sterilized with 0.1% mercuric chloride solution for 10 minutes, rinsed with sterile water for 5 times, and inoculated into the seed germination medium. The success rate of disinfection is 95%. The culture temperature is 24°C, the light intensity is 1500 lx, and the light is 16 hours / day. Seeds germinated after 30 days.

[0022] The seed germination medium is composed of the following components per liter: 20g sucrose, 6g agar, the balance is 1 / 2 MS medium, pH 5.8.

[0023] 2. Adventitious bud induction: 40 days after seed germination, when the seedling height was 1 cm, the hypocotyls were cut and inoculated on the adventitious bud induction medium to induce adventitious buds. The culture tem...

Embodiment 2

[0032] 1. Disinfection method of explant: take the blue-black fruit on the vigorous growth plant of Euryodendron excelsum H.T.Chang, remove the pulp after soaking in water, obtain well-developed seeds, and first soak in 75% ethanol aqueous solution by volume fraction 20 seconds, then sterilized with 0.1% mercuric chloride solution for 8 minutes, rinsed with sterile water for 5 times, and inoculated into the seed germination medium, the success rate of disinfection is 90%. The culture temperature is 26°C, the light intensity is 1800 lx, and the light is 14 hours / day. Seeds germinated after 28 days.

[0033] The seed germination medium is composed of the following components per liter: 25g sucrose, 6g agar, the balance is 1 / 2 MS medium, pH 5.9.

[0034] 2. Adventitious bud induction: 50 days after seed germination, when the seedling height is 1.5 cm, the hypocotyls are cut and inoculated on the adventitious bud induction medium to induce adventitious buds. The culture temperat...

Embodiment 3

[0043] 1. Disinfection method of explant: take the blue-black fruit on the vigorous growth plant of Euryodendron excelsum H.T.Chang, remove the pulp after soaking in water, obtain well-developed seeds, and first soak in 75% ethanol aqueous solution by volume fraction 30 seconds, then sterilized with 0.1% mercuric chloride solution for 5 minutes, rinsed with sterile water 4 times, and inoculated into the seed germination medium, the success rate of disinfection was 85%. The culture temperature is 28°C, the light intensity is 2000 lx, and the light is 12 hours / day. Seeds germinated after 25 days.

[0044] The seed germination medium is composed of the following components per liter: 30g sucrose, 6g agar, the balance is 1 / 2 MS medium, pH 6.0.

[0045] 2. Adventitious bud induction: 60 days after seed germination, when the seedling height was 2 cm, the hypocotyls were cut and inoculated on the adventitious bud induction medium to induce adventitious buds. The culture temperature...

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Abstract

The invention discloses a tissue culture method for euryodendron excelsum. According to the method, hypocotyls of the euryale ferox seeds serve as explants, tissue culture and rapid propagation of high-quality euryale ferox seedlings of excellent strains are successfully conducted, and the produced euryale ferox seedlings have the advantages of being high in hereditary stability, rapid in seedling emergence, strong in seedling, good in seedling quality, high in resistance, good in growth and the like; and good market prospects are realized.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a method for culturing bloodwood tissue. Background technique [0002] Euryodendron excelsum H.T.Chang, the only species of the genus Euryodendron excelsum H.T.Chang in the family Theaceae, is a perennial woody plant. It is currently only produced in Bajia Town, Yangchun County, Guangdong Province. Pig blood wood is a kind of precious wood, and it is also a beautiful ornamental garden seedling. It is on the verge of extinction due to human interference and the destruction of the living environment. At present, it is only distributed in Bajia Town, Yangchun County, Guangdong Province, and the number does not exceed In 1992, it was listed as a national second-class protected endangered plant. In 1998, it was listed as a critically endangered species (Critical Endangered) by the International Plant Conservation Union (IUCN). In 2012, it was listed as a wild plant with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCY02P60/40
Inventor 曾宋君熊玉萍文香英张雪莲陈双艳杨科明吴坤林房林李琳马国华
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI