Construction of stable cell line carrying orthogonal tRNA/aminoacyl tRNA synthetases

A cell line and synthase technology, applied in the field of biopharmaceuticals, can solve problems such as the bottleneck of industrial application of gene codon expansion technology
CN112725282AInactive Publication Date: 2021-04-30PEKING UNIV
7 Cites 5 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
PEKING UNIV
Publication Date
2021-04-30
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a construction method of a stable cell line carrying orthogonal tRNA / aminoacyl tRNA synthetases. On the basis of a gene codon extension technology, a pair of orthogonal tRNA / aminoacyl tRNA synthetases is used for introducing non-natural amino acid into protein at a fixed point. The invention also relates to a construction method of a double lentiviral vector, a construction method of an orthogonal tRNA carrier carrying multiple copy numbers and a method for stably integrating orthogonal tRNA / aminoacyl tRNA synthetase genes into a cell genome by virtue of stable transduction of double slow viruses and stable transfection of plasmids. The invention further relates to the use of the stable cell line, such as the use for the expression of interest proteins containing non-natural amino acids.
Need to check novelty before this filing date? Find Prior Art

Description

[0001] This application is a divisional application of the Chinese patent application No. 2016100555428 with the filing date of January 27, 2016 and the title of the invention being "Construction of a Stable Cell Line Carrying Orthogonal tRNA / Aminoacyl-tRNA Synthetase". technical field

[0002] The invention belongs to the field of biopharmaceuticals and relates to a method for constructing a stable cell line carrying orthogonal tRNA / aminoacyl tRNA synthetase. Background technique

[0003] (1) Gene codon expansion technology

[0004] In recent years, the genetic code expansion technology has developed rapidly. Using the amber stop codon as the sense codon, the designed unnatural amino acid can be introduced into the protein by introducing the corresponding orthogonal tRNA and aminoacyl tRNA synthetase. According to the properties of unnatural amino acids, proteins can be endowed with special functions. So far, this technology has successfully expressed hundreds of unnatural...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More