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Analysis method, spectrometry method and method for identifying organism

An analysis method and analysis method technology, applied in the direction of microbial measurement/inspection, biological testing, analysis of materials, etc., can solve problems such as time-consuming

Pending Publication Date: 2021-05-04
KEIO UNIV +1
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Emm type typing is performed by sequencing the DNA sequence of the emm gene of GAS, but there is a problem that it is time-consuming due to amplification of DNA by PCR (Polymerase Chain Reaction: Polymerase Chain Reaction).

Method used

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  • Analysis method, spectrometry method and method for identifying organism
  • Analysis method, spectrometry method and method for identifying organism
  • Analysis method, spectrometry method and method for identifying organism

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no. 1 Embodiment approach －

[0036] The analysis method of this embodiment acquires information related to emm patterns based on the presence or absence of peaks within a predetermined m / z range in a mass spectrum obtained by mass spectrometry of a sample containing microorganisms.

[0037] (about the sample)

[0038] The sample is not particularly limited as long as it is group A hemolytic streptococcus (GAS) or a sample containing microorganisms that may be GAS. The sample is preferably collected from animals such as humans. When collecting a sample from a human, any body fluid that may contain GAS, such as fluid adhering to the throat or tonsils, exudate from skin wounds, blood, cerebrospinal fluid, and urine, can be used as the sample. For example, in the case of a test for identifying GAS infected by a patient, it is preferable to collect blood from the patient as a sample, or use a throat swab obtained by swabbing the throat of the subject with a cotton swab or the like as a test sample. Sample co...

Embodiment

[0068] Hereinafter, examples of this embodiment will be shown, but the present invention is not limited to specific devices and the like in the following examples.

[0069] GAS of type emm1, type emm12, type emm28, and type emm89 were respectively used as samples, and mass spectra were obtained by MALDI-TOFMS using the AXIMA microbial identification system (Shimadzu Corporation). The matrix used sinapinic acid, and time-of-flight mass analysis was performed in linear mode.

[0070] figure 2 , image 3 as well as Figure 4 It is respectively shown that in the mass spectrum of the sample prepared by the microorganism of emm1 type (emm1.0), the range of m / z is 2000-20000, the range of m / z is 5500-7500 and the range of m / z is 10000-12000 range diagram. The number marked corresponding to the peak is the m / z of the peak, and the ID for identifying the peak is described in parentheses. The same is true for the following diagrams.

[0071] Figure 5 , Image 6 as well as Fi...

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Abstract

An analysis method comprising: obtaining data corresponding to a mass spectrum that is obtained by subjecting a sample containing a microorganism to mass spectroscopy; and obtaining information relating to emm1 group A Streptococcus depending on the presence / absence of a peak or the peak size within a first range wherein the m / z value in the mass spectrum is from 10930 to 10945 inclusive.

Description

technical field [0001] The present invention relates to an analysis method, an analysis method and a microorganism identification method. Background technique [0002] Group A Streptococcus (GAS) is a Gram-positive coccus that causes various symptoms in infected persons. GAS not only becomes the cause of pharyngitis, tonsillitis, etc., but also invades blood or tissue to cause invasive GAS infection. Among invasive GAS infections, those accompanied by shock symptoms are called fatal severe hemolytic streptococcal infections (Streptococcal toxic shock syndrome: STSS). Sometimes in STSS, after the initial symptoms such as limb pain and fever, the condition deteriorates rapidly, causing tissue necrosis and multiple organ failure, etc., resulting in the death of the infected person within a short period of tens of hours. [0003] According to the antigenicity of the M protein on the surface of the bacteria, GAS is classified into more than 130 serotypes and M types. Recently,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/14G01N27/64G01N33/48
CPCC12Q1/14G01N33/6848G01N33/56938G01N33/6851
Inventor 诸角美由纪佐久间惠绪方是嗣岛圭介船津慎治
Owner KEIO UNIV
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