In-vitro life support perfusion culture system and control method thereof

Abstract

The invention relates to the technical field of in-vitro life culture, and discloses an in-vitro life support perfusion culture system, which comprises: a premixing unit, which includes a premixing chamber, and is used for adding a nutrient solution and gas into the premixing chamber, and mixing in the premixing chamber to form a culture solution; a culture unit, which includes a culture chamber, the culture chamber is communicated with the premixing chamber, the culture solution in the premixing chamber can be conveyed into the culture chamber, and the culture chamber is used for containing a culture and culturing the culture; a microscopic observation module, which includes an objective table and an observation assembly, the culture chamber is placed on the objective table, at least one culture chamber can be placed on the objective table, and the observation assembly is used for observing culture in the culture chamber; the microscopic observation module further includes a rack, the objective table is arranged on the rack in a swinging mode, and the objective table can drive the culture chamber to swing synchronously.

Description

technical field [0001] The invention relates to the technical field of in vitro life culture, in particular to an in vitro life sustaining perfusion culture system and a control method thereof. Background technique [0002] Organoids, as a kind of 3D cell tissue, have a very high accuracy rate in the evaluation tools of tumor drugs and drug efficacy evaluation. At present, the culture method of organoids is mainly static culture in a well plate. The operator cuts the obtained primary samples into pieces, then coats them in matrigel at low temperature, and then adds medium and puts them in the incubator for culture. Weekly Perform 3 medium changes and subculture every 7-10 days. There are many problems in this culture mode: first, the growth rate of organoids is slow, and the culture period is long. During the operation, the culture chamber needs to be opened regularly for manual liquid replacement, and the risk of contamination is high; when the number of culture samples in...

AI Technical Summary

Problems solved by technology

There are many problems in this culture mode: First, the growth rate of organoids is slow, and the culture period is long. During the operation, the culture chamber needs to be opened regularly for manual liquid replacement, and the risk of contamination is high; when the number of culture samples increases, it will greatly increase manpower, Equipment, space and other cost input

Second, the static culture in the culture plate will lead to insufficient exchange of organoids and medium, especially the internal nutrients of 3D cell tissue cannot be absorbed, which will eventually limit the growth of organoids, limit the growth degree of organoids and simulate the human body organ similarity

[0003] In order to solve the above technical problems, the prior art adopts perfusion culture, and the culture solution in the culture plate flows out at any time and is drained, but this perfusion culture method cannot be observed during the culture process.

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