Immune cell culture method

A culture method and technology of immune cells, applied in the field of immune cell culture, can solve the problems of low culture efficiency, few cells, complicated manual operation process, etc.

Active Publication Date: 2021-05-11
广东美赛尔细胞生物科技有限公司
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Problems solved by technology

[0003] In the existing immune cell culture, the culture liquid needs to be artificially blown and mixed, and several times of centrifugation are required in the operation process. The manual operation process is complicated, and the culture cost is high. In the existing immune cell culture, the cells cultured at one time Less, the culture efficiency is relatively low, therefore, we propose a immune cell culture method

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Embodiment Construction

[0040] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0041] Such as figure 1 As shown, the present invention provides a method for culturing immune cells, comprising the steps of:

[0042] Step S1: Preparatory work: culture medium (in an incubator at 37°C) and lymphocyte separation medium were rewarmed (at room temperature).

[0043] Step S1: Sample dilution: Dilute the lower layer of erythrocytes after separation of plasma by adding an equal volume of normal saline, fully mix by pipetting and mixing mechanism,...

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Abstract

The invention discloses an immune cell culture method. The method comprises the following steps: S1, preparation work, S2, sample dilution, S3, gradient centrifugation, S4, buffy coat collection, S5, washing, S6, washing, S7, inoculation, S8, addition of feeder cells, S9, culture start, S10, observation and recording, and S11, cryopreservation. According to the immune cell culture method, the conditions that manual operation of cell culture medium blowing is time-consuming and laborious and the blowing effect is poor are avoided, the blowing efficiency is improved, centrifugal treatment of the cell culture medium can be achieved through first centrifugal mechanisms and second centrifugal mechanisms, by arranging four first centrifugal mechanisms and four second centrifugal mechanisms outside a blowing mixing mechanism at one time, multi-station operation is achieved, a large number of immune cells can be cultured at one time, and the device is high in efficiency and is convenient in popularization.

Description

technical field [0001] The invention relates to the technical field of immune cell culture, in particular to a method for culturing immune cells. Background technique [0002] Natural killer cells are important immune cells in the body. They are not only related to anti-tumor, anti-viral infection and immune regulation, but also participate in the occurrence of hypersensitivity and autoimmune diseases in some cases. Cells play an important role in medicine, so immune cells need to be cultivated. [0003] In the existing immune cell culture, the culture liquid needs to be artificially blown and mixed, and several times of centrifugation are required in the operation process. The manual operation process is complicated, and the culture cost is high. Less, the culture efficiency is relatively low, so we propose a method for culturing immune cells. Contents of the invention [0004] The purpose of the present invention is to provide a method for culturing immune cells to sol...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/36C12M1/00
CPCC12M27/04C12M33/10C12M41/00
Inventor 梅建勋欧阳建华汪凯何伟贤付引梅谭慕华梁键全
Owner 广东美赛尔细胞生物科技有限公司
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