Mycoplasma synoviae virulent strain and application thereof

A technology of mycoplasma synovialum and virulent strains, applied in the direction of antibody medical components, bacterial antigen components, medical preparations containing active ingredients, etc., can solve problems such as economic losses, increased mortality rate, and increased waste rate of broiler carcasses

Active Publication Date: 2021-05-11
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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AI Technical Summary

Problems solved by technology

Although Mycoplasma synovialum infection seldom directly causes the death of poultry, the resulting growth retardation, deformed eggshells, increased mortali

Method used

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  • Mycoplasma synoviae virulent strain and application thereof
  • Mycoplasma synoviae virulent strain and application thereof
  • Mycoplasma synoviae virulent strain and application thereof

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Embodiment 1

[0021]Separation, purification, and identification of MS pathogen infected MS using a branch medium, and the establishment of 10 to 11 weeks of age SPF chicken artificial infection model and inactivated vaccine preparation and immunoassay evaluation method, the specific method:

[0022]1. Separation and purification of strains, identification

[0023]Take clinical suspected MS infected chickens, after death, sterile collecting trachea swabs, throat swabs, and airbag swabs, place the sampling swabs directly in aseptic bracitation medium, and send it to the laboratory in time PCR amplification was carried out with MS-specific primers (MS-F: GagaagcTccgtgataTtaAcaaaa) with MS-specific primers (MS-F: GagaagcTccgTgataTtaACAAAAAAACAA). The medium, 37 ° C continued to cultivate, and the color change of the medium on a day, the color was changed from roses to orange red or yellow (figure 1 ), The culture is stored at -70 ° C.

[0024]After the original body isolate is stabilized to 4 generations, mo...

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Abstract

The invention provides a mycoplasma synoviae virulent strain and application thereof. A chicken flock infected by a certain synovial sac in Fujian Province is subjected to MS separation, purification and identification and then named as an MS FZ strain, SPF chickens infected by a pure culture of the MS FZ strain and aged 10-11 weeks have high pathogenicity, and at least 80% of the SPF chickens have air sacculitis; and the MS FZ strain is inactivated through formaldehyde and then emulsified with an oil adjuvant to prepare an inactivated vaccine, the inactivated vaccine is used for immunizing the SPF chickens twice, then a fresh culture of the MS FZ strain is used for counteracting toxic substances to verify the vaccine protection force, and the result shows that at least 80% of the SPF chickens obtain immune protection. Therefore, the separated MS FZ virulent strain can be used as a virulent strain for inspection of effectiveness evaluation of different drugs or prevention and treatment methods and can be used as a vaccine candidate strain for preparing an MS inactivated vaccine.

Description

Technical field[0001]The present invention belongs to the field of biological products, and therefore specifically involves a strong fluid strain and its application thereof.Background technique[0002]Slipocytoplasma (MS) infection mainly causes exudative synovitis, tendon slices of chicken or turkey, but in recent years, the most often occurred in the chicken group is the subclinical upper respiratory tract infection. The bulls of each age can be infected, but with the most vulnerable infection of 28 to 120 days. Different MS strains have different hobbies for different tissues, and the respiratory strain mainly causes subclinical respiratory tract. Disease, mainly airbagitis. Although the infection of the slide gensum is rarely caused by the death of poultry, the growth hysteresis, the eggshell deformity, the increase of deadness, the increase in the increase in the abhydroement of broilers, and the large amount of investment required for prevention and control can cause serious ec...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/04A61K39/02A61P31/04C12R1/35
CPCC12Q1/04A61K39/0241A61P31/04A61K2039/521
Inventor 侯博王晨燕
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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