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A compound polypeptide extract with hypoglycemic and lipid-lowering activity and its application

An extract and mixed extraction technology, applied in the direction of medical preparations containing active ingredients, peptides, drug combinations, etc., can solve the problems of low attention, low degree of commercialization, and low resource utilization of Moringa oleifera leaves, and achieve Good flavor, improve lipid metabolism, lower blood sugar level

Active Publication Date: 2021-07-09
GUANGDONG WENBANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in terms of deep processing of seafood and agricultural products, ginseng and moringa leaves have received little attention, low degree of development, and low resource utilization (ginseng is only used as a cheap catering side dish; moringa leaves are only used as The form of its dry product is circulated on the market); the research on the hypoglycemic and lipid-lowering effects of leaf ginseng peptide and Moringa leaf peptide in vivo has not been reported, and the degree of attention is low, and the degree of commercialization is very low

Method used

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  • A compound polypeptide extract with hypoglycemic and lipid-lowering activity and its application
  • A compound polypeptide extract with hypoglycemic and lipid-lowering activity and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 A compound polypeptide extract with hypoglycemic and lipid-lowering activity

[0047] The preparation steps of the compound polypeptide extract with hypoglycemic and lipid-lowering activity are as follows:

[0048] (1) Dried sea cucumber soaked in warm water: Soak the dried leaf melon in deionized water 20 times the mass of the dried ginseng at a temperature of 50 °C. Soak the wet ginseng in deionized water 20 times the mass of the dry ginseng for 20 min, discard the soaking wastewater, and wash the wet ginseng once more according to the same procedure;

[0049] (2) Grinding wet sea cucumbers: mincing the wet sea cucumbers obtained in step (1) to obtain minced sea cucumbers;

[0050] (3) Enzymatic hydrolysis of sea cucumbers by adding water: mix the minced sea cucumbers obtained in step (2) with deionized water according to the ratio of solid to liquid 1:1 g / mL, stir for 40 min at a stirring speed of 200 r / min, and heat up to 52 °C. Add a compound protease (...

Embodiment 2

[0064] Example 2 A compound polypeptide extract with hypoglycemic and lipid-lowering activity

[0065] The preparation steps of the compound polypeptide extract with hypoglycemic and lipid-lowering activity are as follows:

[0066] (1) Dried sea cucumber soaked in warm water: Soak the dried leaf melon in deionized water 25 times the mass of the dried ginseng at a temperature of 55 °C. After soaking for 12 h, discard the soaking waste water, and use a Soak the wet ginseng in deionized water 25 times the mass of the dry ginseng for 25 minutes, discard the soaking wastewater, and wash the wet ginseng twice again according to the same procedure;

[0067] (2) Grinding wet sea cucumbers: mincing the wet sea cucumbers obtained in step (1) to obtain minced sea cucumbers;

[0068] (3) Enzymatic hydrolysis of sea cucumbers by adding water: mix the minced sea cucumbers obtained in step (2) with deionized water according to the ratio of solid to liquid 1:2 g / mL, stir at a stirring rate o...

Embodiment 3

[0082] Example 3 A compound polypeptide extract with hypoglycemic and lipid-lowering activity

[0083] The preparation steps of the compound polypeptide extract with hypoglycemic and lipid-lowering activity are as follows:

[0084] (1) Dried sea cucumber soaked in warm water: Soak the dried leaf melon in deionized water 30 times the mass of the dried ginseng at a temperature of 60 °C. Soak the wet ginseng in deionized water 30 times the mass of the dry ginseng for 30 min, discard the soaking wastewater, and wash the wet ginseng 3 times in the same procedure;

[0085] (2) Grinding wet sea cucumbers: mincing the wet sea cucumbers obtained in step (1) to obtain minced sea cucumbers;

[0086] (3) Enzymatic hydrolysis of sea cucumbers by adding water: mix the minced sea cucumbers obtained in step (2) with deionized water according to the ratio of solid to liquid 1:3 g / mL, stir at a stirring rate of 400 r / min for 60 min, and heat up to 58 °C. Add a compound protease (purchased fro...

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Abstract

The invention belongs to the technical field of biological peptide preparation, and in particular relates to a compound polypeptide extract with hypoglycemic and lipid-lowering activities and its application. The composite polypeptide extract provided by the invention is prepared by using low edible value sea cucumbers and leaves of Moringa oleifera as raw materials through food processing technology, and the composite polypeptide extract has good blood sugar-lowering and blood-lipid-lowering activities. At the same time, the extraction process of the present invention is simple, and the whole process flow can meet the requirements of food grade, and can be applied in the fields of medicine, health products, food and the like.

Description

technical field [0001] The invention belongs to the technical field of biological peptide preparation, and in particular relates to a compound polypeptide extract with hypoglycemic and lipid-lowering activities and its application. Background technique [0002] Diabetes is a chronic systemic metabolic disease related to genetic factors and various environmental factors. Type 2 diabetes is the most complex and has the highest prevalence rate, accounting for more than 90% of the patients in my country. At present, there are about 110 million diabetic patients in my country, which is the country with the largest number of diabetic patients in the world. Diabetes usually causes hyperlipidemia, and hyperlipidemia can also lead to the development of diabetes. Therefore, diabetes combined with hyperlipidemia is very common, and the harm it causes is also very large. However, mild hyperlipidemia usually has no discomfort, so it is often ignored. Therefore, the development of healt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/34C07K1/30C07K1/36A61K38/01A61P3/10A61P3/06A23L33/18A23L2/39A23L2/52
CPCA23L2/39A23L2/52A23V2002/00A61K38/011A23L33/18A61P3/06A61P3/10C07K1/14C07K1/145C07K1/30C07K1/34C07K1/36C12P21/06
Inventor 林恋竹赵强忠赵谋明
Owner GUANGDONG WENBANG BIOTECHNOLOGY CO LTD
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