Fluorescence quenching restorer and application thereof

Abstract

The invention discloses a fluorescence quenching restorer and application thereof, and relates to the field of immunoassay and biotechnology application. The fluorescence quenching restorer is prepared from the following components in parts by volume, 35-45 parts of absolute ethyl alcohol and 55-65 parts of PBS (Phosphate Buffer Solution), and the application of the fluorescence quenching restorer comprises the following steps of A1, cleaning a fluorescence-quenched tissue slice or cell sample by using the PBS solution to obtain a fluorescence quenching restorer solution; wherein the cleaning time is 1-2 times, and each time is 5 minutes; A2, dropwise adding the fluorescence quenching restorer on a tissue slice or a cell sample, and incubating at room temperature for 10 minutes; and A3, cleaning the tissue slice or the cell sample with a PBS solution for 3 times, 5 min each time, and obtaining the tissue slice or the cell sample after fluorescence quenching recovery. The method is advantaged in that immunofluorescence sample with fluorescence quenching can be re-dyed, so the fluorescence of the re-dyed immunofluorescence sample can be well displayed, the error-tolerant rate of an immunofluorescence experiment can be increased, the number of samples can be reduced, and the experiment time can be saved.

Description

Technical field[0001]The present invention relates to immunoassays and biotechnology applications, and more particularly to fluorescent quenching recovery agents and their applications.Background technique[0002]Immunofluorescence is often one-time, that is, once, it is observed with a fluorescent microscope or laser co-focus. However, if the immunofluorescence incubation fluorescent secondary resistance can cause fluorescence quenching, for example, the illumination of white light or other causes can only make up the mistakes by re-experiment. If the experimental sample is precious, for example, the tissue samples that are rare diseases or the experimental samples obtained for a long time, there is often no excess sample to make mistakes. At the same time, for precious samples, you want to detect multiple indicators with immunofluorescence, and existing immunofluorescence techniques can only detect five colors, but also subject to detection of instruments, which leads to the require...

AI Technical Summary

Problems solved by technology

At the same time, for precious samples, I want to use immunofluorescence to detect multiple indicators, but the existing immunofluorescence technology can only detect five colors at most, and is limited by the number of detection channels of the detection instrument, which leads to the required sample size Increase, prolong the experiment time

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