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Method for utilizing humic acid in pig farm biogas slurry by nano titanium dioxide reinforced microalgae

A nano-titanium dioxide, humic acid technology, used in microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc.

Inactive Publication Date: 2021-05-18
SHANGRAO NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on the purification of pig farm biogas slurry by photocatalytic coupling with microalgae

Method used

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  • Method for utilizing humic acid in pig farm biogas slurry by nano titanium dioxide reinforced microalgae

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Experimental program
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Effect test

example

[0020] Step 1: Nearly Neptona spinosa ( Desmodesmus sp. CHX1) were inoculated into the medium and cultured for 3 days to enter the logarithmic phase;

[0021] Step 2: Wash the Algae paraspinosa in step 1 with pure water and inoculate it into an Erlenmeyer flask containing 400 mL of pig farm biogas slurry. Initial UV in pig farm biogas slurry 254 0.44, the initial inoculum concentration of microalgae was 0.4 g / L (chlorophyll was 0.04 mg / L), the culture temperature was 30°C, and the light intensity was 250 μmol photons m -2 ·s -1 , the light-dark cycle is 12h / 12h, and the shaker speed is 150rpm. After 2 hours of treatment, UV in pig farm biogas slurry 254 35%; after 36 hours of cultivation, the concentration of microalgae chlorophyll reached 2.6mg / L.

Embodiment 2

[0023] Step 1: Nearly Neptona spinosa ( Desmodesmus sp. CHX1) were inoculated into the medium and cultured for 3 days to enter the logarithmic phase;

[0024] Step 2: Wash the C. spinosa in step 1 with pure water and inoculate it into an Erlenmeyer flask containing 400mL of pig farm biogas slurry, add 0.2g / L nano-titanium dioxide and place it in a light incubator nourish. Initial UV in pig farm biogas slurry 254 is 0.44, the initial inoculum concentration of microalgae is 0.4g / L (chlorophyll is 0.04mg / L), the culture temperature is 30°C, and the light intensity is 250μmol photons m -2 ·s -1 , the light-dark cycle is 12h / 12h, and the shaker speed is 150rpm. After 2 hours of treatment, UV in pig farm biogas slurry 254 After 36 hours of cultivation, the concentration of microalgae chlorophyll reached 6.5mg / L, which was 3.9 mg / L higher than that of the control treatment.

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Abstract

The invention relates to a method for utilizing humic acid in pig farm biogas slurry by nano titanium dioxide reinforced microalgae. The humic acid which is difficult to absorb and utilize by the microalgae in the pig farm biogas slurry is decomposed into micromolecular organic acid or CO2 which can be absorbed and utilized by the microalgae through nano titanium dioxide, and the purpose of improving the absorption and utilization effect of the microalgae on the humic acid is achieved. The process parameters are as follows: the initial inoculation concentration of microalgae is 0.1-0.4 g / L, the addition amount of nano titanium dioxide is 0.01-0.3 g / L, the culture temperature is 20-40 DEG C, the illumination intensity is 250-610 [mu] mol photons.m <-2>. s <-1>, the light-dark period is 12 h / 12 h, the rotating speed of a shaking table is 150 rpm, and the culture time is 2-72 h. At the moment, the chlorophyll content of the microalgae and the removal rate of humic acid in the wastewater are 2.5 times and 3.3 times of those of control treatment respectively, the technological process is simple and convenient, and large-scale popularization is easy.

Description

technical field [0001] The invention relates to a method for using nano-titanium dioxide to strengthen microalgae to utilize humic acid in pig farm biogas slurry, and specifically belongs to the technical field of wastewater treatment. Background technique [0002] my country is a big pig raising country. The rapid development of pig raising industry has resulted in a large amount of pig raising wastewater. Water pollution has become an important bottleneck restricting the development of pig raising industry in my country. At present, large-scale pig farms at home and abroad mainly use anaerobic fermentation system to treat pig wastewater. Although the system can reduce the concentration of organic matter in pig wastewater and produce biogas, it also produces a large amount of pig farm biogas. The content of nutrients such as nitrogen and phosphorus in pig farm biogas slurry is very high, and the carbon-nitrogen ratio is seriously out of balance, which is not conducive to b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/38C02F3/32C12R1/89C02F103/20
CPCC12N1/12C12N1/38C02F3/322C02F2103/20
Inventor 罗龙皂林小爱胡可馨王慧敏刘林海廖兴李苗
Owner SHANGRAO NORMAL UNIV