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Application of interaction of banana MuMADS1 and MaMADS55 in regulation and control of MaGWD1 gene expression

A gene expression and gene technology, applied in the biological field, to improve quality and promote up-regulation of expression

Active Publication Date: 2021-05-25
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether the MuMADS1 transcription factor and MaMADS55 transcription factor can interact with the MaGWD1 gene and regulate its expression has not been reported yet.

Method used

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  • Application of interaction of banana MuMADS1 and MaMADS55 in regulation and control of MaGWD1 gene expression
  • Application of interaction of banana MuMADS1 and MaMADS55 in regulation and control of MaGWD1 gene expression
  • Application of interaction of banana MuMADS1 and MaMADS55 in regulation and control of MaGWD1 gene expression

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Embodiment Construction

[0029] Referring to the accompanying drawings, the present invention will be further described in conjunction with specific embodiments, so as to better understand the present invention. If no specific technique or condition is indicated in the examples, it shall be carried out according to the technique or condition described in the literature in this field or according to the product specification. The reagents or instruments used were not indicated by the manufacturer, and they were all commercially available conventional products.

[0030] 1 Experimental materials

[0031] (1) Bananas (M. acuminata L. AAA group cv Brazilian) were obtained from the banana plantation of the Institute of Tropical Biotechnology (20N, 110E, Chiang Mai, Hainan Province, China), and were transported to the laboratory immediately after harvest. Select a healthy fruit comb and divide it into individual fruit fingers. Select intact and undamaged fruit fingers and sterilize the surface with 0.1% sod...

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Abstract

The invention provides an application of interaction of banana MuMADS1 and MaMADS55 in regulation and control of expression of a MaGWD1 gene. The banana transcription factors MuMADS1 and MaMADS55 are adopted for the first time, can be combined with a banana MaGWD1 promoter region, interact with the MaGWD1 gene and regulate and control the expression of the MaGWD1 gene. For example, a transcription factor MuMADS1 gene and / or a transcription factor MaMADS55 gene and a banana MaGWD1 promoter are introduced into banana fruits, up-regulation expression of the MaGWD1 gene can be remarkably promoted, a VIGS silencing system is constructed by adopting the transcription factor MuMADS1 gene and / or the transcription factor MaMADS55 gene, expression of the MaGWD1 gene can be remarkably inhibited, reduction of starch content can be effectively inhibited, glucan-water double kinase activity can be effectively inhibited, and meanwhile, increase in the content of fructose, glucose, cane sugar and the like in the banana fruits is effectively inhibited.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an application of the interaction between banana MuMADS1 and MaMADS55 in regulating the expression of MaGWD1 gene. Background technique [0002] Banana (Musa acuminata) plays a central role in the development of society and agricultural economy. Banana is a globally important fruit and economic crop, accounting for 16% of global fresh fruit production (Dale et al., 2017). And starch is the In general, the starch content in the banana fruit can reach 20% to 25% of the fresh weight (DoNascimento et al., 2006) when harvesting. Banana is a common starch-transformed fruit, and the starch in the banana fruit ripens after harvesting rapidly. Degraded and converted into soluble sugar, its conversion efficiency directly affects the firmness, sweetness and shelf life of the fruit (Saraiva et al., 2013).Therefore, elucidating the molecular mechanism of starch degradation in postharv...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/113C12N15/82A01H5/08A01H6/00
CPCC07K14/415C12N15/8218C12N15/8245
Inventor 刘菊华罗劲梅金志强刘梦婷徐碧玉王静毅贾彩红苗红霞张建斌王卓
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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