Intelligent gas-phase anti-oxidation film containing rosemary temperature-sensitive lipidosome and preparation method thereof
A temperature-sensitive lipid and rosemary technology, applied in the direction of protein coating, chitin polysaccharide coating, hyaluronic acid coating, etc., can solve the long-term effect of anti-oxidation active packaging film and temperature sensitivity, poor practical application effect, etc. problem, to achieve the effect of obvious temperature-sensitive characteristics and obvious slow-release effect
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[0023] An intelligent gas-phase antioxidant film containing rosemary thermosensitive liposomes, which is prepared by the following method:
[0024] S1. To prepare rosemary thermosensitive liposomes, dipalmitoylphosphatidylcholine (DPPC, Shanghai Aladdin Biochemical Technology Co., Ltd.) 100mg, cholesterol (CH, biochemical reagents, Sinopharm Chemical Reagent Co., Ltd.) 20mg and Rosemary essential oil (REO, reagent grade, American ChromaDex company) 10mg was dissolved in 7.8L ether (anhydrous ether, analytical grade, Sinopharm Chemical Reagent Co., Ltd.) to obtain mixed solution 1, and the mixed solution was mixed under stirring conditions Add PBS dropwise to 100ml of PBS buffer solution at 30°C, and continue to stir for 30 minutes after the dropwise addition to obtain mixed solution 2; transfer mixed solution 2 to a round-bottomed flask, and use a rotary evaporator to remove diethyl ether to obtain the mixed solution three, and the mixed solution three was ultrasonically passe...
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