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Sampling brush suitable for sampling microorganisms on solid surface and sampling head

A microbial sampling and solid surface technology, applied in biological material sampling methods, enzymatic/microbiological devices, specific-purpose bioreactors/fermentation tanks, etc., can solve inaccurate test results, limited sample collection, inability to Effective collection of samples and other issues to achieve effective collection and consistent efficiency

Pending Publication Date: 2021-05-25
BEIJING CENT FOR PHYSICAL & CHEM ANALYSIS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the method of using cotton swabs for microbial sampling on solid surfaces is only for local sampling of solid surfaces, and the sample collection volume is very limited; at the same time, the sampling size plate used in this method can only be used for sampling samples on flat solid surfaces, and the sampling of solid surface pits is limited. Depressed and uneven samples have limitations; when the level of bacteria or virus contamination on the solid surface is low, the effective collection of samples cannot be carried out, which will inevitably lead to inaccurate test results

Method used

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  • Sampling brush suitable for sampling microorganisms on solid surface and sampling head
  • Sampling brush suitable for sampling microorganisms on solid surface and sampling head
  • Sampling brush suitable for sampling microorganisms on solid surface and sampling head

Examples

Experimental program
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Effect test

Embodiment 1

[0054] This embodiment provides a sampling brush suitable for sampling microorganisms on solid surfaces, which can be adapted to sampling complex surfaces, and can also effectively collect samples for samples with potholes and uneven solid surfaces.

[0055] like Figure 1-Figure 4 As shown, this sampling brush comprises: bristle 191, bristle bracket 192 and quick interface 193; The end face is arrayed with multiple rows of bristles 191, and the bristles 191 are made of bristle sheath 195 wrapping bristle fiber bundle 196 (bristle fiber bundle 196 stretches out the bristle sheath 195 to set the length); wherein the bristle sheath 195 is a polymer material with elasticity; The bristle fibers forming the bristle fiber bundle 196 are materials with water absorption and water delivery functions, such as cotton fibers, and the elasticity of the bristle sheath 195 enables the sampler to perform sampling on complex surfaces with ups and downs on solid surfaces. One end of the bristl...

Embodiment 2

[0059] Based on the sampling brush in Embodiment 1 above, this embodiment provides a translational sampling head, which is an automatic sampling mechanism capable of sampling at a constant rate.

[0060] like Figure 6-Figure 9 As shown, the translational sampling head includes: a driving mechanism A, a traveling mechanism, a travel limit assembly and a sampling assembly A; wherein the driving mechanism A is used to provide power for the traveling mechanism; the traveling mechanism is used to drive the sampling assembly to move within the sampling area ; The travel limit component is used to limit the travel of the traveling mechanism; the sampling component is used to realize sampling. specific:

[0061] The driving mechanism A includes: a driving motor A8, a fixed connecting rod A9 and a motor control signal transmission line;

[0062] The traveling mechanism includes: gear A7, rack A21, gear B13, rack B15, traveling wheel A23, guide rail A16, traveling wheel B17, guide ra...

Embodiment 3

[0083] This embodiment provides a rotary sampling head, and the sampling head adopting this structural form can cope with emergent biosafety incidents and implement emergency large-scale screening and sampling.

[0084] like Figure 10-Figure 13 As shown, the rotary sampling head includes: a driving mechanism and a sampling assembly; in order to distinguish from Embodiment 2, the driving mechanism in this embodiment is the driving mechanism B; the sampling assembly is the sampling assembly B;

[0085] The sampling assembly B is a rotary component, including: a sampling brush bracket 121 and a plurality of sampling brushes 19; the sampling brushes 19 are the sampling brushes in the first embodiment above. The sampling brush bracket 121 is a disc structure, and a plurality of sampling brushes 19 are distributed uniformly along the circumferential direction around the axis of the sampling brush bracket 121 on the lower end surface of the sampling brush bracket 121; The bracket 1...

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Abstract

The invention discloses a sampling brushsuitable for sampling microorganisms on a solid surface and a sampling head , and the sampling brush and the sampling head can adapt to sampling on a complex surface and can also effectively collect samples with hollow and uneven solid surfaces. The sampling brush comprises bristles and a bristle bracket, the bristle bracket is of a hollow structure, and a liquid storage material is contained in a cavity of the bristle bracket. The bristles are arranged on the lower end face of the bristle bracket, the bristles are formed by wrapping bristle fiber bundles with bristle outer skins, and the bristle fiber bundles extend out of the bristle outer skins by a set length; the outer skin of the bristles is made of an elastic material; brush hair fibers forming the brush hair fiber bundles are materials with water absorption and water delivery functions; one end of the bristles is implanted into the cavity of the bristle bracket and is in contact with the liquid storage material; and more than one vent hole is formed in the upper end surface of the bristle bracket. In addition, based on the sampling brush, the invention further provides a translation type sampling head and a rotary type sampling head.

Description

technical field [0001] The invention relates to a sampling brush and a sampling head, in particular to a sampling brush and a sampling head suitable for sampling microorganisms on a solid surface, belonging to the microorganism detection and sampling technology. Background technique [0002] Routine solid surface microbial sampling generally adopts the surface smear method. The national standards "GB4789.17-2003" and "GB / T18204.4-2013" stipulate the solid surface microbial sampling method, perform aseptic operation on the solid surface, use sterilized dry cotton swabs, and place on the appropriate part of the solid surface or certain Smear the area evenly back and forth for sample collection, and then use sterilized scissors to cut off the head of the cotton swab and put it into the buffer for shaking and eluting. The shaking eluent is the collected microbial sample on the solid surface. [0003] However, the method of using cotton swabs for microbial sampling on solid surf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/26C12M1/00
CPCC12M1/00C12M1/26
Inventor 张裕祥刘清珺杜美红
Owner BEIJING CENT FOR PHYSICAL & CHEM ANALYSIS
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