Walnut peptide stable to zinc ions as well as preparation method and application of walnut peptide
A technology of walnut peptide and zinc ion, applied in the application, the function of food ingredients, the processing of vegetable protein, etc., can solve the problems of solution mixing and affecting the sensory quality of products, etc.
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Embodiment 1
[0048] (1) 50kg of walnut protein powders are configured into 1000kg of protein solutions with a concentration of 5% by mass with deionized water, and the walnut protein powders are dispersed at a shear rate of 500 rpm;
[0049] (2) Heat the dispersed walnut protein solution to 90°C and keep it for 120 minutes to fully expand the molecular structure of the walnut protein, and then cool it to 60°C;
[0050] (3) Add 0.025kg tannase (Xiasheng (Beijing) Biotechnology Development Co., Ltd., food grade tannase FDG-2254), 0.025kg cellulase (Xiasheng (Beijing) Biological Science and Technology Development Co., Ltd., food-grade solid cellulase FDG-2226), enzymolysis at 50°C for 2 hours, degrades polyphenols, cellulose and other components in walnut protein powder, reduces discoloration, and is conducive to further enzymolysis;
[0051] (4) Use a concentration of 1mol L -1 Sodium hydroxide solution to adjust the pH of the enzymolysis solution finally obtained in step (3) to 7.0~9.0, add ...
Embodiment 2
[0060] (1) 200kg of walnut protein powder is configured into 1000kg of protein solution with a concentration of 20% by mass with deionized water, and the walnut protein powder is dispersed at a shear rate of 500 rpm;
[0061] (2) Heat the dispersed walnut protein solution to 95°C and keep it for 5 minutes to fully expand the walnut protein molecular structure, and then cool the temperature to 60°C;
[0062] (3) Add 0.1kg tannase (Xiasheng (Beijing) Biotechnology Development Co., Ltd., food grade tannase FDG-2254), 0.1kg cellulase (Xiasheng (Beijing) Biological Science and Technology Development Co., Ltd., food-grade solid cellulase FDG-2226), enzymolysis at 50°C for 2 hours, degrades polyphenols, cellulose and other components in walnut protein powder, reduces discoloration, and is conducive to further enzymolysis;
[0063] (4) Use 10mol·L -1 potassium hydroxide solution to adjust the pH of the enzymolysis solution finally obtained in step (3) to 7.0-9.0, add 4kg alkaline pro...
Embodiment 3
[0071] (1) 100kg of walnut protein powder is configured into 1000kg of protein solution with a concentration of 10% by mass with deionized water, and the walnut protein powder is dispersed at a shear rate of 5000 rpm;
[0072] (2) Heat the dispersed walnut protein solution to 95°C and keep it for 30 minutes to fully expand the walnut protein molecular structure, and then cool it to 60°C;
[0073] (3) Add 0.1kg tannase (Xiasheng (Beijing) Biotechnology Development Co., Ltd., food grade tannase FDG-2254), 0.1kg cellulase (Xiasheng (Beijing) Biological Science and Technology Development Co., Ltd., food-grade solid cellulase FDG-2226), enzymolysis at 50°C for 2 hours, degrades polyphenols, cellulose and other components in walnut protein powder, reduces discoloration, and is conducive to further enzymolysis;
[0074] (4) Use 5mol·L -1 Calcium hydroxide solution to adjust the pH of the enzymolysis solution finally obtained in step (3) to 7.0-9.0, add 2kg alkaline protease (Alcalas...
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