Method for strengthening recycled aggregate concrete by utilizing microbial denitrification phenomenon and recycled aggregate concrete
A technology of recycled aggregate and denitrification, applied in the field of construction waste recycling, can solve the problems of high crushing value, high water absorption, low apparent density of recycled aggregate, etc., and achieve the effect of low cost
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preparation example 1
[0070] This preparation example provides a bacterial liquid of denitrifying bacteria, which is prepared by the following preparation method:
[0071] (a) Inoculate the denitrifying bacteria on the culture medium and culture on a shaker at 30°C for 18 hours. When the OD value reaches 1, the bacterial concentration can reach 10 9 CFU / mL;
[0072] Described culture medium is: the American BD234000 culture medium of 5g / L, the sodium chloride of 5g / L and distilled water prepare bacterial liquid culture medium, and culture medium is carried out high temperature and high pressure steam sterilization;
[0073] (b) Centrifuge to obtain bacteria, add distilled water to prepare 10 7 cfu / mL bacterial solution.
preparation example 2
[0075] This preparation example provides a kind of bacterium liquid of denitrifying bacteria, and the difference of preparation example 1 is only, step (b) adds distilled water and is mixed with 10 5 cfu / mL bacterial solution.
preparation example 3
[0077] This preparation example provides a kind of bacterium liquid of denitrifying bacteria, and the difference of preparation example 1 is only, step (b) adds distilled water and is mixed with 10 8 cfu / mL bacterial solution.
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