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Method for synthesizing bimodal nano-hydroxyapatite by taking nucleic acid aptamer as template

A technology of nano-hydroxyapatite and nucleic acid aptamers, which is used in pharmaceutical formulations, MRI/MRI contrast agents, preparations for in vivo tests, etc., achieves clear imaging, excellent physical and chemical properties, and promotes research and development. Effect

Active Publication Date: 2021-06-01
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Compared with the traditional rare earth-doped hydroxyapatite synthesis method, its synthesis method has the characteristics of simple steps, mild synthesis conditions (reaction at room temperature), and high rare earth doping efficiency, which solves the problem of constructing existing nano-hydroxyapatite imaging probes. The complexity of the material and the limitation of imaging sensitivity by factors such as material agglomeration

Method used

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  • Method for synthesizing bimodal nano-hydroxyapatite by taking nucleic acid aptamer as template
  • Method for synthesizing bimodal nano-hydroxyapatite by taking nucleic acid aptamer as template
  • Method for synthesizing bimodal nano-hydroxyapatite by taking nucleic acid aptamer as template

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Embodiment 1

[0027]The present embodiment is simply synthesized by a nucleic acid adapter as a template, and is doped with a rare earth ion having an imaging function therein, thereby obtaining dual modal nanocroxyphosphite having cancer cell targeting identification function. . We pass nucleic acid fittings AS1411 and CA2+The electrostatic adsorption effect such that NHAP is synthesized in AS 1411 to be achieved, and a rare earth ion having a fluorescent imaging function and MRI imaging function is doped by NHAP ion exchange characteristics. Simple and fast implementation of the specific process of synthesizing bispecium nanocroxypholite in a nucleic acid suitable ligand as a template is (the total volume of the reaction system is 1 mL, the Moore concentration of the AS1411 in the system is 50 μmol / L, CA2+Molar concentration 50mmol / L, PO43-Moore concentration 30mmol / L, GD3+Moore concentration 7.5mmol / L, TB3+Moore concentration 7.5mmol / L, F-Molar concentration 3mmol / L):

[0028]1) The A...

Embodiment 2

[0032]The elemental composition of the AS1411-NHAP: Gd / Tb synthesized in Example 1 was investigated, and the elemental composition of the synthetic material was measured by transmitting electron microscope (EDS) and X-ray photoelectron energy spectrometry (XPS).figure 2 Indicated.

[0033]Fromfigure 2 It can be seen that the synthesized AS1411-NHAP: GD / TB contains elements Ca, O, P, F, GD, TB, which proves successful synthesis of dual modal NHAP.

Embodiment 3

[0035]The same method is used in Example 1, only adjusts AS1411 and CA2+The molar concentration ratio is 1: 500, 1: 1: 1: 2000, 1: 5000, 1: 10000, and AS1411, which is the same as in Example 1, prepared for the corresponding AS1411-NHAP: GD / TB sample and no Control samples of AS1411. Experiment As1411-NHAP: GD / TB and control samples in different AS1411 and CA2+The zeta potential and particle size of the molar concentration ratio are measured by the nanoparture potentiometer, as a result,image 3 andFigure 4 (A) shown. Fromimage 3 It can be seen that as the AS1411 molar concentration increases, the potential is made of positive and negative, which can prove the successful modification of the AS1411; the larger the absolute value of the zeta potential, the better the nanomaterial stability, when AS1411 and CA2+When the molar concentration ratio is 1: 1000, NHAP is already in a stable state;Figure 4 (A) shows when AS1411 and CA2+When the molar concentration ratio is 1: 1: 1: 1: 500,...

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Abstract

The invention discloses a method for synthesizing bimodal nano-hydroxyapatite by taking a nucleic acid aptamer as a template. Rare earth ions with an imaging function are doped in the process of synthesizing nano-hydroxyapatite by taking the nucleic acid aptamer as the template, and the nucleic acid aptamer is AS1411. The rare earth ions with the imaging function are Tb<3+> with the fluorescence imaging function and Gd<3+> with the MRI imaging capability. A biomimetic synthesis method with a nucleic acid aptamer as a template is adopted, synthesis of nHAp capable of specifically recognizing cells / tissues is simply and rapidly achieved, rare earth ions with different imaging functions are doped in the synthesis process, and preparation of the fluorescent / MRI nano imaging probe (AS1411-nHAp: Gd / Tb) is achieved. The nano material has the characteristics of good biocompatibility, excellent physical and chemical properties, high rare earth ion doping rate, diversified functions and the like.

Description

Technical field[0001]The present invention relates to a method of synthesizing a rare earth ion-doped diblant nanocroxypholite in a mold in a nucleic acid fit.Background technique[0002]Nano-Hydroxyapatite, NHAP is the main composition of the human and animal mineralization, such as bone, teeth and calcified tendons, with excellent biocompatibility, biological activity, and biological affinity, Inducing bone tissue regeneration, has been widely used in bone defect repair and bone tissue engineering. Its crystal structure and surface characteristics give its powerful ion exchange capacity, complexing capacity, and adsorption. As the pharmaceutical carrier, it has been widely used in the treatment of cancer, osteoporosis, osteoitis and other diseases. In addition, NHAP also has anti-tumor activity, which has inhibitory effect on tumor cells such as hepatoma cells, intestinal cancer cells, intestinal cancer cells, intestinal cancer cells, can inhibit cancer cell proliferation, promote c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K49/08A61K49/10
CPCA61K49/0019A61K49/08A61K49/005A61K49/10A61K49/0052
Inventor 周蓉卉张文青李婷
Owner SICHUAN UNIV
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