Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for synthesizing bimodal nano-hydroxyapatite using nucleic acid aptamer as a template

A nano-hydroxyapatite and nucleic acid aptamer technology is applied in pharmaceutical formulations, nuclear magnetic resonance/magnetic resonance imaging contrast agents, preparations for in vivo experiments, etc., to achieve clear imaging, small crystal particle size, and cell entry efficiency. high effect

Active Publication Date: 2022-03-15
SICHUAN UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the traditional rare earth-doped hydroxyapatite synthesis method, its synthesis method has the characteristics of simple steps, mild synthesis conditions (reaction at room temperature), and high rare earth doping efficiency, which solves the problem of constructing existing nano-hydroxyapatite imaging probes. The complexity of the material and the limitation of imaging sensitivity by factors such as material agglomeration

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for synthesizing bimodal nano-hydroxyapatite using nucleic acid aptamer as a template
  • A method for synthesizing bimodal nano-hydroxyapatite using nucleic acid aptamer as a template
  • A method for synthesizing bimodal nano-hydroxyapatite using nucleic acid aptamer as a template

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] In this example, nano-hydroxyapatite is simply and quickly synthesized by using nucleic acid aptamer as a template, and doped with rare earth ions with imaging function, so as to obtain bimodal nano-hydroxyapatite with cancer cell target recognition function . We use the nucleic acid aptamer AS1411 and Ca 2+ The electrostatic adsorption effect enables the synthesis of nHAp using AS1411 as a template, and through the ion exchange characteristics of nHAp, it is doped with rare earth ions with fluorescence imaging and MRI imaging functions. The specific process for the simple and rapid synthesis of bimodal nano-hydroxyapatite using nucleic acid aptamers as templates is as follows (the total volume of the reaction system is 1 mL, the molar concentration of AS1411 in the system is 50 μmol / L, Ca 2+ Molar concentration of 50mmol / L, PO 4 3- The molar concentration of 30mmol / L, Gd 3+ The molar concentration of 7.5mmol / L, Tb 3+ The molar concentration of 7.5mmol / L, F - The ...

Embodiment 2

[0032] Investigate the elemental composition of AS1411-nHAp:Gd / Tb synthesized in Example 1, measure the elemental composition of the synthetic material by transmission electron microscopy (EDS) and X-ray photoelectron spectroscopy (XPS), the results are as follows figure 2 shown.

[0033] From figure 2 It can be seen that the synthesized AS1411-nHAp:Gd / Tb contains elements Ca, O, P, F, Gd, and Tb, which proves the successful synthesis of bimodal nHAp.

Embodiment 3

[0035] Using the same method as in Example 1, only adjusting AS1411 and Ca 2+ The molar concentration ratios are 1:500, 1:1000, 1:2000, 1:5000, 1:10000 and AS1411-free respectively. Others are the same as in Example 1, and corresponding AS1411-nHAp:Gd / Tb samples and AS1411-nHAp:Gd / Tb samples without Control sample of AS1411. Investigate AS1411-nHAp:Gd / Tb and control samples in different AS1411 and Ca 2+ The Zeta potential and the particle size under the molar concentration ratio are measured by a nanometer particle size potentiometer, and the results are as follows image 3 and Figure 4 (A) shown. From image 3 It can be seen that with the increase of the molar concentration of AS1411, the potential changes from positive to negative, which can prove the successful modification of AS1411; the greater the absolute value of Zeta potential, the better the stability of nanomaterials, when AS1411 and Ca 2+ When the molar concentration ratio of the nHAp is 1:1000, nHAp is alrea...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for synthesizing dual-mode nano-hydroxyapatite using nucleic acid aptamers as templates. The rare earth ion, the nucleic acid aptamer is AS1411, and the rare earth ion with imaging function is Tb with fluorescence imaging function 3+ and Gd with MRI imaging capability 3+ . The present invention adopts the biomimetic synthesis method using the nucleic acid aptamer as a template to simply and quickly realize the synthesis of nHAp with specific cell / tissue recognition, and dope rare earth ions with different imaging functions in the synthesis process to realize fluorescence / MRI nano-imaging probe (AS1411‑nHAp:Gd / Tb), nanomaterials have the characteristics of good biocompatibility, excellent physical and chemical properties, high doping rate of rare earth ions, and diversified functions.

Description

technical field [0001] The invention relates to a method for one-step synthesis of dual-mode nano-hydroxyapatite co-doped with rare earth ions by using nucleic acid aptamer as a template. Background technique [0002] Nano-hydroxyapatite (nano-Hydroxyapatite, nHAp) is the main component of the inorganic phase in human and animal mineralized tissues such as bones, teeth and calcified tendons, and has excellent biocompatibility, bioactivity and bioaffinity, and can Inducing bone tissue regeneration has been widely used in the field of bone defect repair and bone tissue engineering. Its crystal structure and surface properties endow it with strong ion exchange capacity, complexation capacity and adsorption capacity. As a drug carrier, it has been widely used in the treatment of cancer, osteoporosis, periostitis and other diseases. In addition, nHAp also has anti-tumor activity, can inhibit the activity of tumor cells such as liver cancer cells, intestinal cancer cells, osteos...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K49/08A61K49/10
CPCA61K49/0019A61K49/08A61K49/005A61K49/10A61K49/0052
Inventor 周蓉卉张文青李婷
Owner SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com