Antibody pair for detecting canine distemper virus and application thereof

Abstract

The invention discloses an antibody pair for detecting canine distemper virus and application of the antibody pair, and belongs to the technical field of biology. The invention relates to an antibody pair for detecting canine distemper virus, which comprises an antibody 1A4 heavy chain CDR-H1 to CDR-H3, namely SEQ ID NO: 1 -3, and an antibody 1A4 light chain CDR-L1 - CDR-L3, namely SEQ ID NO: 4 - 6; the heavy chain CDR-H1-CDR-H3 of the antibody 1B2 is shown in SEQ ID NO: 7-9; the light chains CDR-L1-CDR-L3 of the antibody 1B2 are shown in SEQ ID NO: 10 - 12. The antibody pair can specifically recognize the canine distemper viruses, 1000 TCID50 / mL canine distemper viruses can be detected at least in an antigen test strip experiment, 400 TCID50 / mL canine distemper viruses can be detected at least in a double-antibody sandwich method ELISA experiment, the recognition specificity is high, and the antibody pair does not react with other viruses.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an antibody pair for detecting canine distemper virus and its application. Background technique [0002] Canine distemper (Canine distemper) is infected by canine distemper virus (CDV), which causes a disease accompanied by immunosuppression and systemic infection. Infected dogs can cause symptoms of the respiratory tract, digestive tract, and central nervous system. The infection group is quite extensive, including panda dogs, mink, foxes, wolves, terrestrial carnivores, and aquatic mammals such as whales and seals. Canine distemper virus belongs to the Paramyxoviridae family, a member of the genus Morbillivirus, a negative-strand RNA virus with an envelope structure and no segments. The total length of the virus genome is about 15690bp, and the virus particles are mostly spherical. The length of the nucleoprotein located in the protein envelope is 600-800 nanometers, a...

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Problems solved by technology

[0003] With the rapid development of the current pet diagnosis and treatment industry, more and more attention has been paid to the diagnostic technology of canine distemper based on immune response (Xu Gang. Screening of canine distemper virus monoclonal antibody and establishment of double antibody sandwich ELISA[D].Northwest University of Agriculture and Forestry Science and Technology, 2019.), and monoclonal antibodies are important reagents for diagnosing antigens, and a monoclonal antibody with neutralizing effect (CN 102618503B) has been developed (Bi Zhenwei, Xu Libo, Xia Xingxia, Liang Ting, Wang Yongshan. Identification Preparation of neutralizing monoclonal antibodies against strong and weak strains of canine distemper virus [J]. Jiangsu Agricultural Science, 2020, 48(20): 178-182.) However, there is a lack of relevant reports on the development of canine distemper paired monoclonal antibodies. The monoclonal antibody group can form a sandwich structure for antigen detection, which is the key to the development of sandwich ELISA and antigen colloidal gold test strips; in this study, two high-affinity monoclonal antibody strains 1A4 and 1B2 were screened by purifying canine distemper virus , detected by Western blot, the size of its recognition of canine distemper protein is about 63kDa, it is presumed to recognize the F protein of canine distemper virus. After testing, this paired antibody can be used in the development of sandwich ELISA reagents or colloidal gold reagents

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