Method for preparing feline distemper virus monoclonal antibody by using bioreactor

A monoclonal antibody and bioreactor technology, applied in biochemical equipment and methods, chemical instruments and methods, microorganisms, etc., can solve the problems of mice being susceptible to other diseases, unable to scale production, and increasing economic costs, and achieve The production process and quality are controllable, the production cost is reduced, and the effect of improving product quality

Pending Publication Date: 2021-06-01
哈尔滨元亨生物药业有限公司
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Problems solved by technology

The concentration of antibodies collected by this method is high, but the operation is cumbersome, the yield is low, and large-scale production cannot be performed. Mice are susceptible to other diseases, and there will be a large amount of impurity prot

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  • Method for preparing feline distemper virus monoclonal antibody by using bioreactor
  • Method for preparing feline distemper virus monoclonal antibody by using bioreactor

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[0041] The technical solution of the present invention will be further described below in conjunction with the accompanying drawings, but it is not limited thereto. Any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be covered by the present invention. within the scope of protection.

[0042] The invention provides a method for preparing feline distemper virus monoclonal antibody by using a bioreactor. The cells used in the method are mouse hybridoma cells, which are derived from Beijing Century Yuanheng Animal Epidemic Prevention Technology Co., Ltd., and the serum-free medium is purchased from Beimenji Biotechnology Co., Ltd., cell culture shaker was purchased from American Jingqi Co., Ltd., DMEM medium was purchased from Thermo Fisher Scientific (China) Co., Ltd., and bovine serum was purchased from Lanzhou Rongye Co., Ltd.

[0043...

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Abstract

The invention discloses a method for preparing a feline distemper virus monoclonal antibody by using a bioreactor. The method comprises the following steps: step 1, carrying out subcloning on hybridoma cells by using a limited dilution method, selecting single cells into a serum-free culture medium, and carrying out suspension culture to obtain a cell strain A805 with high survival rate and strong antibody secretion ability, wherein the preservation number of the strain is CGMCC No.21902; step 2, preparing anti-feline distemper virus monoclone by using a bioreactor, wherein hybridoma cells are cultured in two stages, namely a cell growth stage and an antibody secretion stage, in the bioreactor; and step 3, harvesting the cell culture fluid as an antibody, and centrifuging to remove cell debris, thereby obtaining a finished product of the feline distemper virus monoclonal antibody. According to the method, the titer of the distemper virus monoclonal antibody is improved, the defects of a traditional ascites production antibody are overcome, and the antibody product is free of impure protein, stable in physicochemical property and small in batch-to-batch difference.

Description

technical field [0001] The invention relates to a preparation method of feline distemper virus monoclonal antibody. Background technique [0002] Feline distemper is also called feline panleukopenia, feline infectious gastroenteritis (Enteritis infectiosa felum) or feline ataxia syndrome. In 1928, Verge and Cristoforoni proved that the disease was caused by a virus. In 1930, Hammon and Ender first reported the isolation of feline distemper virus. In 1984, a strain of feline distemper virus was first isolated from natural cases in my country. [0003] Feline panleukopenia virus (Feline panleukopenia virus) is a member of the Parvoviridae family and the genus Parvovirus. In addition to infecting domestic cats, the virus can also infect animals such as tigers, leopards, minks, and raccoons. Young cats are mostly acutely ill, manifested as high fever, vomiting, diarrhea and other symptoms, and the mortality rate is as high as 90% in severe cases. Vaccination against feline di...

Claims

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Application Information

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IPC IPC(8): C12N5/20C07K16/08
CPCC12N5/163C07K16/081C12N2500/90
Inventor 孙博麻昌姣叶阳任德强张健张立恒宋新刚井鸿博郭婷
Owner 哈尔滨元亨生物药业有限公司
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