Cationic polypeptide-heat shock protein-miRNA gene compound as well as preparation method and application thereof

A heat shock protein and cationic polypeptide technology, which is applied in the field of cationic polypeptide-heat shock protein-miRNA gene complexes, achieves good development and application prospects, inhibits growth, and improves survival effects.

Inactive Publication Date: 2021-06-04
AFFILIATED YONGCHUAN HOSPITAL OF CHONGQING MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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However, the intracellular delivery of these macromolecular drugs is a great challenge

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  • Cationic polypeptide-heat shock protein-miRNA gene compound as well as preparation method and application thereof
  • Cationic polypeptide-heat shock protein-miRNA gene compound as well as preparation method and application thereof
  • Cationic polypeptide-heat shock protein-miRNA gene compound as well as preparation method and application thereof

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Embodiment Construction

[0022] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0023] 1. Preparation of cationic polypeptide-heat shock protein-miRNA gene complex

[0024] 1. Design and synthesis of cationic peptides

[0025] The amino acid sequence of the cationic polypeptide designed in the present invention is shown in SEQ ID No.1. Meanwhile, the ovalbumin (OVA) epitope peptide is used as a control peptide, and its amino acid sequence is shown in SEQ ID No.2.

[0026] Polypeptides were synthesized on an ABI 431A solid-phase peptide synthesizer (PE, USA). Methods The st...

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Abstract

The invention discloses a cationic polypeptide-heat shock protein-miRNA tumor gene compound as well as a preparation method and application thereof. The compound is formed by combining cationic polypeptide, heat shock protein Grp78 and miR-125a through non-covalent bonds, and the compound can be recognized by antigen presenting cells, induce powerful anti-tumor immunity and effectively inhibit growth of tumor cells; and the compound is simple to prepare, can be used for preparing tumor therapeutic gene compounds, and has good development and application prospects in the field of tumor treatment.

Description

technical field [0001] The invention relates to a gene complex, in particular to a cationic polypeptide-heat shock protein-miRNA gene complex, and also relates to a preparation method and application of the complex. Background technique [0002] In the treatment of malignant tumors, surgery, chemotherapy and radiotherapy have been the main methods for a long time, but they all have their limitations, such as low surgical resection rate, high postoperative recurrence rate that cannot be predicted and controlled, and radiotherapy and chemotherapy can cause severe immune damage. system damage, etc. It is imminent to seek a safe, effective and non-invasive treatment method for malignant tumors. Studies in recent years have shown that cell gene deletion and mutation lead to out-of-control cell growth and malignant transformation are the main mechanisms of malignant tumors. The etiology of tumors mainly includes activation of oncogenes, mutation inactivation of tumor suppressor ...

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Application Information

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IPC IPC(8): A61K38/16A61K48/00A61K31/7088A61K47/64A61P35/00
CPCA61K38/16A61K31/7088A61K47/64A61P35/00
Inventor 杨曌
Owner AFFILIATED YONGCHUAN HOSPITAL OF CHONGQING MEDICAL UNIV
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