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An integrated closed nucleic acid automatic extraction device

An automatic extraction and closed technology, applied in solvent extraction, separation methods, sugar derivatives, etc., can solve the problems of low integration, cross-contamination, and large volume of nucleic acid extraction drive devices, so as to achieve wide application, avoid cross-contamination, Effects suitable for rapid prototyping and verification work

Active Publication Date: 2022-05-03
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the technical problems of the existing nucleic acid extraction drive device, which are large in volume, low in integration, difficult to realize modularization, and easy to cause cross-contamination, and further provide an integrated closed nucleic acid automatic extraction device

Method used

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  • An integrated closed nucleic acid automatic extraction device
  • An integrated closed nucleic acid automatic extraction device
  • An integrated closed nucleic acid automatic extraction device

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specific Embodiment approach 2

[0057] Specific implementation mode two: as Figure 2-Figure 4 As shown, this embodiment is a further description of the specific embodiment one. The end cap 1 is a cylindrical structure, and the top of the end cap 1 is provided with a plurality of through holes 1-1 (through holes 1-1 The number is preferably three, and the angle between the centers of every two adjacent through holes 1-1 is 120°), and the bottom of the end cap 1 is provided with an inner cavity 1-2 (the inner cavity 1-2 is used to fix the long reagent container Cavity 3).

specific Embodiment approach 3

[0058] Specific implementation mode three: as figure 1 , Figure 31 and Figure 32 As shown, this embodiment is a further description of the second specific embodiment. The long flexible reagent tube 2 and the short flexible reagent tube 4 are both cylindrical cavities (and both are straw-shaped structures), and the long flexible reagent tubes The reagent tube 2 and the short flexible reagent tube 4 are used to accommodate the reaction reagent. The top of the long flexible reagent tube 2 and the short flexible reagent tube 4 have an air pressure balance port and a reagent hole at the bottom.

[0059] Specific implementation mode four: as figure 1 , Figure 5-Figure 7 As shown, this embodiment is a further description of the third specific embodiment. The long reagent chamber 3 is a cylindrical structure, and the upper end surface of the long reagent chamber 3 is provided with a plurality of blind holes-3-1( The quantity of blind hole one 3-1 is preferably three, and the in...

specific Embodiment approach 7

[0062] Specific implementation mode seven: as figure 1 , Figure 16-Figure 18 As shown, this embodiment is a further description of specific embodiment 1. The vertical flow channel 7 is a cylindrical structure, and the vertical flow channel 7 is provided with a central through hole 7-2, and the central through hole 7- 2 is a stepped hole (the diameter of the upper end of the stepped hole is greater than the diameter of the lower end), and the middle part of the outer circumferential surface of the vertical flow channel 7 is provided with the annular shoulder 7-1 (for positioning); the vertical conduit 10 is arranged on In the central through hole 7-2 of the vertical channel 7.

[0063] Specific implementation mode eight: as Figure 19-Figure 21 As shown, this embodiment is a further description of Embodiment 1. The upper part of the mixing chamber 8 is a thin-walled casing 8-1, and the lower part is a thin-walled hemispherical structure 8-2; the vertical flow The lower end ...

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Abstract

An integrated closed nucleic acid automatic extraction device belongs to the technical field of nucleic acid extraction. The lower end of the vertical channel is tightly fitted in the mixing chamber, the upper end of the vertical channel is tightly fitted in the thin-walled circular tube at the lower end of the horizontal flow channel, and the upper end of the horizontal flow channel is tightly fitted in the lower thin-walled casing of the short reagent chamber. The lower end of the reagent chamber is tightly fitted in the upper thin-walled casing of the short reagent chamber, and the upper end of the long reagent chamber is tightly covered with an end cap; a vertical conduit reaching the bottom of the mixing chamber is arranged in the vertical channel, and the long reagent chamber A plurality of long soft reagent tubes are arranged in a plurality of blind holes in the chamber, and a plurality of short soft reagent tubes are arranged in a plurality of blind holes in the short reagent cavity; the long soft reagent tubes and the short soft reagent Shape memory alloys are respectively fixed on the outside of the tube. The invention adopts a closed structure to avoid cross-contamination of reagents and products, and the invention is used for nucleic acid extraction.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid extraction, and in particular relates to an integrated closed automatic nucleic acid extraction device. Background technique [0002] Nucleic acid is the main object of molecular biology research. Nucleic acid extraction is the most basic and very important process in molecular biology experimental techniques. Many studies use the nucleic acid extraction process as the initial step. Therefore, rapid, convenient and high-purity nucleic acid extraction has become an important research content, and high-throughput, automated nucleic acid extraction methods will also facilitate in vitro diagnosis. [0003] Manual extraction of nucleic acids in traditional laboratories requires strict division of areas, and has corresponding requirements for the experimental environment. Operators need to use pipettes and test tubes to continuously manually inject, pipette, mix, and drain the reagents used in nucl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D11/00C07H1/00C07H21/02
CPCB01D11/00C07H1/00C07H21/02
Inventor 李松晶吕斯宁
Owner HARBIN INST OF TECH