NCoV-N protein detection kit and nCoV-N protein detection method
A protein detection, ncov-n technology, applied in the field of immunology detection, can solve the early stage of viral infection, the human body may not have produced antibodies and other problems
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Embodiment 1
[0057] The nCoV-N protein detection kit described in this embodiment specifically comprises the following steps:
[0058] 1) Solution preparation
[0059] Buffer A preparation: 50mM boric acid-borax buffer solution (pH8.0), 0.5% Triton, 1% BSA, 2% sucrose.
[0060] Buffer B preparation: 20mM Na 3 PO 4 , 5% BSA, 0.25% Tween-20 and 10% sucrose in distilled water.
[0061] Buffer C preparation: 0.1% Triton-100, PBS preparation.
[0062] Preparation of buffer D: 20mM Tris-HCL, pH 8.0, 500mM NaCl and 10% glycerol, prepared in distilled water.
[0063] Preparation of buffer E: 20mM Tris-HCl, pH 8.0, 20mM imidazole, 500mM NaCl and 10% glycerol, prepared in distilled water.
[0064] Preparation of buffer F: 20mM Tris-HCl, pH 8.0, 200mM imidazole, 500mM NaCl and 10% glycerol, prepared in distilled water.
[0065] Buffer G preparation: 20mM Tris-HCl, pH 8.0, 10mM EDTA, 5mM MgCl 2 and 10% glycerin in distilled water.
[0066] 2) Prepare the construction of the protein gene recomb...
Embodiment 2
[0093] This embodiment is the determination of the sensitivity and specificity of the nCoV-N protein detection kit prepared in Example 1
[0094] Dilute healthy human serum and patient serum with sample diluent to different concentrations: 100 times, 50 times, 10 times samples, and put them into the sample pad of the biosensor. See results within 5 minutes.
[0095] Test results such as figure 2 As shown, from left to right, the biosensors are loaded with serum from healthy people and 100-fold, 50-fold, and 10-fold diluted serum from patients. The results showed that only the control line (C line) developed color in the serum test results of healthy people; the patient serum diluted 100 times was positive at a low concentration, the C line developed color, and the control line (T line) had lighter color development; 50 times the patient's serum is positive, the C line is colored, and the T line is more obvious; the serum of the patient diluted 10 times is positive, the C li...
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Abstract
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