Biological 3D printing method for large tissues

Abstract

The invention relates to a biological 3D printing method for large tissues. A prepared sacrificial material and vascularized cells are placed in a syringe to obtain a first mixed solution and a first gel material corresponding to the first mixed solution;a prepared matrix material is mixed with tissue cells to obtain a second mixed solution and a second gel material corresponding to the second mixed solution, the first gel material and the second gel material are loaded into a 3D printer, and an outlet of the 3D printer is connected to a coaxial printing spray head; the coaxial printing spray head starts printing according to a preset path track and according to a to-be-printed organ file, extruded gel fibers are gelatinized on a refrigeration platform, matrix material components in the gel fibers are irreversibly cured, and a printing structure is obtained; the printing structure is immersed in a culture medium at a second set temperature, a hollow flow channel structure is formed, and a network structure with vascular cells is obtained; and the vascular network structure is subjected to dynamic culture, reconstruction of a vascular network in the structure is promoted, and therefore a vascularized three-dimensional tissue structure capable of keeping effectiveness of a flow channel is obtained.

Description

technical field [0001] The invention relates to the technical field of biomanufacturing, in particular to a method for bio-3D printing of bulk tissues. Background technique [0002] In recent years, 3D printing technology has gradually been used in tissue engineering and regenerative medicine to solve the problem of lack of organ donors. The idea of ​​traditional tissue engineering technology is to plant the corresponding cells on the pre-printed biological scaffolds, and carry out in vitro simulation of in vivo culture or transplantation into the body. Compared with traditional tissue engineering, bio-3D printing technology mixes cells in bio-ink when printing tissues or organs. In this way, the cell density can be achieved, and the spatial distribution of multiple types of cells in different structural materials can be controlled. Therefore, bioprinting technology, also known as cell printing and organ printing, has gradually become a research hotspot in the manufacture o...

AI Technical Summary

Problems solved by technology

Compared with the traditional printing process, the multi-functional coaxial biological structure manufacturing process based on the gel tubular unit provides a better printing unit idea on the printing unit during the printing process, but because the structural strength of the hollow tube is often not enough Support the entire structure, causing the structure to collapse and the effectiveness of the runners to be affected

In addition, the original printing method requires secondary perfusion of endothelial and other vascularized functional cells for the reconstruction of vascularization inside the printed tissue, which is relatively cumbersome.

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