Method for detecting melamine in milk

A technology of melamine and detection method, which is applied in the direction of material analysis by observing the influence on chemical indicators, and analysis by chemical reaction of materials, etc., can solve the complicated operation steps, high cost of equipment and instruments, and difficult commercial application question

Pending Publication Date: 2021-06-08
JINGGANGSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these existing technologies all have common disadvantages: the operation steps are cumbersome and the cost of equipment and instruments used is high, making it difficult to carry out commercial application

Method used

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  • Method for detecting melamine in milk
  • Method for detecting melamine in milk

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The present embodiment provides a kind of detection method of melamine in milk, comprising the following steps:

[0027] S1: Mix 2 mL of trichloroacetic acid with a mass fraction of 10% and 5 mL of milk to be tested to obtain a mixed solution A. Mix the mixed solution A ultrasonically at a frequency of 78 Hz for 20 minutes and then stir for 10 minutes;

[0028] S2: Centrifuge the mixed solution A of S1 at a speed of 10,000 rpm for 15 minutes to obtain a supernatant, and then use 1mol / L NaOH solution to adjust the pH value of the supernatant to 7. After the adjustment, the volume of the supernatant is 3 mL;

[0029] S3: Add 30 μL of gallic acid and 30 μL of 1mol / L sodium hydroxide solution to 3 mL of the supernatant in sequence and stir evenly to prepare mixed solution B;

[0030] S4: Add 60 μL of 10 μmol / L silver nitrate solution to the mixed solution B of S3 to obtain the mixed solution C, then transfer it to a cuvette with a thickness of 2 cm, and let it stand for 15 ...

Embodiment 2

[0036] The raw material used in this embodiment and mode of operation are all identical with embodiment 1, difference is:

[0037] In S1, the ultrasonic frequency is 130KHZ, the ultrasonic mixing time is 18min, and the stirring time is 8min;

[0038] In S2, the centrifugation speed is 12000rpm, the centrifugation time is 13min, and the pH value of the supernatant is adjusted to 8;

[0039] In S4, the standing time is 13min;

[0040] In b, the standing time is 13 minutes.

Embodiment 3

[0042] The raw material used in this embodiment and mode of operation are all identical with embodiment 1, difference is:

[0043] In S1, the ultrasonic frequency is 25KHZ, the ultrasonic mixing time is 22min, and the stirring time is 12min;

[0044] In S2, the centrifugation speed is 8000rpm, the centrifugation time is 17min, and the pH value of the supernatant is adjusted to 9;

[0045] In S4, the standing time is 17min;

[0046] In b, the standing time is 17 minutes.

[0047] Now choose embodiment 1 as preferred embodiment, it is tested to illustrate effect of the present invention.

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Abstract

The invention belongs to the field of food detection, and particularly relates to a method for detecting melamine in milk, which comprises the following steps: S1, mixing trichloroacetic acid with the mass fraction of 10% and milk to be detected according to the volume ratio of (2-2.2):5 to obtain a mixed solution A, and uniformly mixing; S2, centrifuging the mixed solution A in the S1, taking supernatant liquid, and then adjusting the pH value of the supernatant liquid to 7-9; S3, sequentially adding 9.8-10 [mu]mol / L gallic acid with the volume being 1 / 1000-1 / 1100 of the volume of the supernatant and a 0.98-1 mol / L sodium hydroxide solution with the volume being 1 / 1000-1 / 1100 of the volume of the supernatant into the supernatant in the S2, and uniformly mixing to prepare a mixed solution B; S4, adding a 9.8-10 [mu]mol / L silver nitrate solution which is 2-2.5 times of the volume of gallic acid in S3 into the mixed solution B in S3 to obtain a mixed solution C, then transferring the mixed solution C into a cuvette, and standing; and S5, comparing the mixed solution C after standing with a colorimetric solution, and determining the molar concentration of melamine in the mixed solution C. The detection method provided by the invention can simply and quickly detect melamine in milk, and is very economical and efficient.

Description

technical field [0001] The invention belongs to the field of food detection, and in particular relates to a method for detecting melamine in milk. Background technique [0002] The nitrogen content of melamine is as high as 66%, so it is often added to milk, animal feed and other products to increase its protein content. However, excessive intake of melamine can cause kidney stones or lead to renal failure or even death in infants. Therefore, in the commercial production of milk, it is necessary to measure the melamine content of the finished milk, so as to prevent unqualified milk with excessive melamine content from flowing into the market and causing damage to the health of consumers. [0003] Existing melamine detection methods include high performance liquid phase method, potentiometric titration method and gas chromatography method, etc., with a wide variety and different technical solutions. However, these existing technologies all have common disadvantages: the ope...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 刘雪霞王娟邓卫珍刘利民王志军
Owner JINGGANGSHAN UNIVERSITY
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