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Fungus for inhibiting growth of pyricularia grisea and blocking melanin secretion of pyricularia grisea

A technology of rice blast fungus and melanin, which is applied in the field of microorganisms, can solve the problems of non-inhibitory bio-control bacteria and achieve obvious effects of growth inhibition

Pending Publication Date: 2021-06-11
MUDANJIANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies in the prior art, the present invention provides a fungus that inhibits the growth of Magnaporthe grisea and blocks its melanin secretion, and further expands to inhibit the melanin secretion of most pathogenic fungi, solving the problem of biocontrol without inhibiting the melanin secretion of plant pathogenic fungi. bacteria problem

Method used

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  • Fungus for inhibiting growth of pyricularia grisea and blocking melanin secretion of pyricularia grisea
  • Fungus for inhibiting growth of pyricularia grisea and blocking melanin secretion of pyricularia grisea
  • Fungus for inhibiting growth of pyricularia grisea and blocking melanin secretion of pyricularia grisea

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Embodiment

[0028] As shown in the figure, the embodiment of the present invention provides a fungus that inhibits the growth of Magnaporthe grisea and blocks its melanin secretion, including the following specific experimental methods:

[0029] Step 1. Analyzing biocontrol bacteria (No. SS7) on the growth of Magnaporthe grisea and the inhibitory effect of melanin secretion by plate confrontation culture;

[0030] Step 2. Analyzing the biocontrol bacteria (No. SS7) metabolic liquid on the growth of Magnaporthe grisea and the inhibitory effect of melanin secretion by PDA solid culture;

[0031] Step 3. Analyze the inhibitory effect of the biocontrol bacteria (SS7) metabolic fluid on the growth of Magnaporthe grisea and the secretion of melanin by PDA liquid culture.

[0032] Step 1: Analyze the biocontrol bacteria (SS7 No.) on the growth of Magnaporthe grisea and the inhibitory effect of melanin secretion by plate confrontation culture:

[0033] Select a petri dish with the same specifica...

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Abstract

The invention provides a fungus for inhibiting growth of magnaporthe oryzae and blocking melanin secretion of the magnaporthe oryzae, and relates to the technical field of microorganisms. The method for analyzing the effect of the biocontrol bacterium for inhibiting the growth of the magnaporthe oryzae comprises the following specific experimental methods: step 1, analyzing the inhibiting effect of the biocontrol bacterium (SS7 #) on the growth amount of the magnaporthe oryzae and the secretion of melanin through plate confrontation culture; 2, analyzing the inhibition effect of the biocontrol bacterium (SS7 #) metabolite on the growth amount of magnaporthe oryzae and melanin secretion through PDA (potato dextrose agar) solid culture; and step 3, analyzing the inhibition effect of the biocontrol bacterium (SS7 #) metabolite on the growth of pyricularia grisea and the secretion of melanin through PDA liquid culture. The biological bacterium (SS7 #) can obviously inhibit and even block melanin secretion of magnaporthe oryzae, in a PDA plate bacteriostasis test, the biocontrol bacterium has an obvious growth inhibition effect on the magnaporthe oryzae and has an obvious inhibition effect on melanin secretion of the magnaporthe oryzae, and when the addition amount of a biocontrol bacterium metabolite in a PDA culture medium reaches 75%, not only is the growth of the magnaporthe oryzae inhibited, but also the the secretion of melanin can be completely blocked.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a fungus that inhibits the growth of blast fungus and blocks its melanin secretion. Background technique [0002] Plant fungal diseases are one of the three major diseases in plant cultivation (bacterial diseases, fungal diseases, and viral diseases), which occur in various cultivation areas in the world. The disease can cause different degrees of production reduction, and even crop failure in severe cases. The main conditions for the cycle and serious occurrence of plant fungal diseases are initial infection and re-infection. The basis of initial infection is the number of pathogenic bacteria that can survive the winter safely; the basis of re-infection is that conditions are suitable for rapid reproduction after initial infection. Among plant pathogenic fungi, there are many bacteria that secrete melanin during the growth process, such as Pyricularia oryzae in rice blast...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12Q1/18C12N1/14C12R1/645
CPCC12Q1/04C12Q1/18C12N1/14G01N2333/37
Inventor 律凤霞张彦丽李艳萍王美玲
Owner MUDANJIANG NORMAL UNIV