miRNA marker for diagnosing cardiovascular diseases and application of miRNA marker

Abstract

According to the miRNA marker for cardiovascular disease diagnosis and the application of the miRNA marker, (1) 100mu mol / l Hcy intervenes endothelial cells to establish an apoptosis model, and cell viability staining and flow cytometry are used for detecting that the concentration Hcy can cause endothelial cell apoptosis, so that a foundation is laid for subsequent experiments; (2) after the step (1), the expression level of the miR-491-5p is detected by RT-qPCR (Reverse Transcription-Quantitative Polymerase Chain Reaction); it is determined that miR-491-5p plays a role in endothelial cell apoptosis caused by Hcy; (3) after the miR-491-5p mimics and the miR-491-5p inhibitor are transfected, the expression level of the miR-491-5p is detected through RT-qPCR; after the fact that the miR-491-5p mimics and the miR-491-5p inhibitor are successfully transfected is determined, a foundation is laid for reversely verifying the effect of the miR-491-5p on the endothelial cell apoptosis caused by the Hcy; and (4) miR-491-5p mimics and miR-491-5p inhibitor are respectively transfected, the cell apoptosis condition is detected again by flow cytometry, and it is determined that the miR-491-5p acts on the endothelial cell apoptosis caused by Hcy. The miR-491-5p plays an important role in endothelial cell injury caused by Hcy, the invnetion provides a theoretical basis for detecting and diagnosing atherosclerosis at a molecular level in the future, and has a potential practical value.

Description

Technical field [0001] The invention belongs to the field of medical technology, and in particular, there is a miRNA marker for cardiovascular disease diagnosis and its application. Background technique [0002] Cardiovascular Disease (CVD) is a global incidence and the highest mortality. At present, the number of patients with cardiovascular disease in my country is 290 million, and mortality ranks first in all diseases, accounting for more than 40% of the death of residents. The pathogenesis of CVD is not fully understood. Its risk factors mainly include high blood pressure, diabetes, hypoxia, and old age, etc. Most cardiovascular diseases are related to the development of Atherosclerosis, AS. In recent years, with the improvement of people's living standards, the changes in food habits, the increase in work pressure, and the changes of social environment, AS The incidence rate has a significant increase, and the age is increasingly ganghua, thus early detection, preventing and...

AI Technical Summary

Problems solved by technology

At present, the treatment of atherosclerosis is mostly drug therapy. Common drugs include aspirin and clopidogrel, but the side effects of drugs are relatively large, causing allergic reactions, liver damage, kidney damage, gastrointestinal reactions, and blood system toxicity. The applicable population is small and cannot be widely popularized; carotid artery ultrasound and angiography, which are the gold standard for detecting vascular lesions, are only diagnostic for atherosclerosis that has undergone structural changes in arteries, and it is difficult to directly observe atherosclerosis that has not yet undergone structural changes. Atherosclerotic lesions; and angiography is traumatic, especially for patients who are allergic to contrast media and have poor renal function

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