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Broiler chicken abdominal fat percentage molecular marker LPIN1 g.256 and detection method and application thereof

A technology of molecular markers and abdominal fat rate, applied in the field of poultry genetics and breeding, can solve the problems of less research and mining of molecular markers, high cost of molecular markers, short generation interval, etc., and achieve the effect of low cost, high accuracy and easy operation

Active Publication Date: 2021-06-11
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reason is that, on the one hand, there are few molecular markers research and excavation on the economic traits of chickens, and the molecular markers that can be applied to select important economic traits in actual breeding are insufficient; on the other hand, the detection of molecular markers is often costly The cost of applying molecular marker-assisted selection technology in chicken breeding with short and large breeding groups is higher than that of conventional breeding methods

Method used

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  • Broiler chicken abdominal fat percentage molecular marker LPIN1 g.256 and detection method and application thereof
  • Broiler chicken abdominal fat percentage molecular marker LPIN1 g.256 and detection method and application thereof
  • Broiler chicken abdominal fat percentage molecular marker LPIN1 g.256 and detection method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 SNP site screening

[0022] 1. Subjects

[0023] With the selection standard of abdominal fat rate, select high abdominal fat rate group (recorded as H group) and low abdominal fat rate group (recorded as L group) two tail samples in five varieties, the selected variety and the two samples in each variety. The number of tail samples is as follows: 12 Guangxi Lingshan chickens, 12 bantam yellow chickens, 12 Jiangfeng ephedra chickens, 10 Zhicheng Xinghua chickens, and 10 recessive white-feathered Rock chickens.

[0024] Table 1 shows the statistics of the determination data of traits such as abdominal fat rate of the two-tailed samples of each variety. In the two tail samples of Lingshan chicken in Guangxi, there was no significant difference in live weight between group H and group L (P>0.05), and the average abdominal fat weight (173.25g) of group H was about 37.58g higher than that of group L (37.58g). 4.61 times, the average abdominal fat rate of group H ...

Embodiment 2

[0041] Embodiment 2 time-of-flight mass spectrometry analysis

[0042] The candidate SNP sites obtained by direct sequencing were genotyped in 500 N409 (Guangxi Lingshan chicken) populations using the time-of-flight mass spectrometry typing technology of Beijing Liuhe Huada Gene Technology Co., Ltd., and the SNP typing was performed by SPSS 21.0 software The results were combined with the slaughter traits related to abdominal fat (abdominal fat rate, abdominal fat weight), and the SNPs significantly correlated with the abdominal fat rate of yellow feather broilers were identified, as shown in Table 3 below.

[0043] Table 3 SNPs information table

[0044]

Embodiment 3

[0045] Example 3 Difference analysis between genotypes of SNPs significantly correlated with abdominal fat rate

[0046]Through SPSS 21.0 software, the analysis results of different genotypes in the SNP locus significantly correlated with abdominal fat rate, abdominal fat rate, abdominal fat weight, subcutaneous fat thickness and other traits are shown in Table 4.

[0047] Table 4 Difference analysis of genotypes of SNPs significantly correlated with abdominal fat rate

[0048]

[0049]

[0050] Note: The number in parentheses after the genotype indicates the number of samples of the genotype; the same letter among the three genotypes indicates no significant difference (P>0.05), different letters indicate significant difference (P<0.05), and no letter indicates the gene genotype was not significantly different from the other two genotypes.

[0051] Therefore, LPIN1 gene marker-assisted selection can be used to select broiler chickens with low abdominal fat rate in broi...

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Abstract

The invention discloses a broiler chicken abdominal fat percentage molecular marker LPIN1g.256 as well as a detection method and application thereof, and relates to the technical field of poultry genetic breeding. The invention provides SNP related to the abdominal fat percentage of a broiler chicken. The sequence of the SNP is shown as SEQIDNO: 1. The invention also provides a specific primer group for detecting the SNP and a specific detection method, which can efficiently, sensitively and quickly detect the SNP sequence so as to screen and identify the broiler variety with low abdominal fat rate. By implementing the SNP, the low-abdominal-fat-rate broilers can be effectively screened, a new technical scheme is provided for genetic breeding of the broilers, and the SNP is suitable for large-scale popularization and application in the broiler breeding industry.

Description

technical field [0001] The invention relates to the technical field of poultry genetics and breeding, in particular to a broiler abdominal fat rate molecular marker LPIN1g.256 and a detection method and application thereof. Background technique [0002] The source of the unique flavor of chicken is mainly intramuscular fat and subcutaneous fat. On the one hand, tenderness and juiciness in meat quality indicators come from the dissolution of muscle fibers by intramuscular fat; on the other hand, the aroma produced by rich phospholipids during the lipid degradation process of intramuscular fat and subcutaneous fat It is an important part of the chicken flavor. At the same time, the unsaturated fatty acids in the muscle undergo oxidation reaction when heated, and the oxidation products are further decomposed into volatile substances such as alkenal, which is an important component of the chicken flavor. The unique flavors of various famous local breed chickens in my country, s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2531/113Y02P60/87
Inventor 罗庆斌张细权聂庆华张德祥罗文李恒丰李红梅叶茂詹惠娜
Owner SOUTH CHINA AGRI UNIV