Multi-channel refrigerating device for microtube electrophoretic separation system and working method thereof

Abstract

The invention relates to a multi-channel refrigerating device for a microtube electrophoretic separation system and a working method thereof. The multi-channel refrigerating device comprises a separation channel arranged on a substrate and a component collecting channel vertically communicated with the separation channel, a first binding post and a second binding post are arranged at the two ends of the separation channel respectively, and a capillary tube used for sample injection is communicated with the separation channel between the component collection channel and the second binding post; and a third binding post and a fourth binding post are respectively arranged at two ends of the component collecting channel. During use, different high voltages and maintaining voltages are connected through the first binding post, the second binding post, the third binding post and the fourth binding post, Joule heat generated in the separation channel is dissipated in time, the temperature and the conductivity of a buffer solution running in the separation channel are reduced, then voltage and current changes caused by the Joule heat are reduced, and sample component separation is achieved. The device has the advantages of improving the separation efficiency and stabilizing current and voltage.

Description

technical field [0001] The invention relates to the technical field of electrophoresis separation, in particular to a multi-channel refrigeration device for a microtube electrophoresis separation system and a working method thereof. Background technique [0002] Under the action of an electric field, the charged particles move toward the opposite electrode, which is called electrophoresis. The technique of using charged particles to move at different speeds in an electric field to achieve separation is called electrophoresis. [0003] Capillary electrophoresis (CE), also known as high-efficiency capillary electrophoresis, is a new technology for efficient and rapid separation of ions or charged particles in a capillary with a high-voltage DC electric field as the driving force. [0004] In the existing capillary electrophoresis separation technology, there are the following problems: (1) the capillary easily generates Joule heat during the electrophoresis process. The prior...

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Problems solved by technology

[0004] In the existing capillary electrophoresis separation technology, there are the following problems: (1) the capillary easily generates Joule heat during the electrophoresis process. The prior art usually adopts a built-in cooling system to take away the Joule heat generated during the electrophoresis process. The cooling system tends to fail to dissipate the Joule heat in the capillary in time, and the Joule heat accumulates in the capillary, which causes the temperature of the running buffer at both ends of the capillary to rise, the conductivity to increase, and the voltage and current to deviate from their linear relationship; (2) due to The limitation of the capillary diameter makes the sample load small, resulting in low efficiency of electrophoretic separation, and cannot achieve high-efficiency separation

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