Electrochemical immunosensor of electroactive substance modified MOF composite material as well as preparation and application of electrochemical immunosensor

Abstract

The invention relates to an electrochemical immunosensor of an electroactive substance modified MOF composite material as well as preparation and application of the electrochemical immunosensor. According to the electrochemical immunosensor, a Cu < 2 + > modified metal-organic framework composite material and a [Fe (CN) 6] < 3-> modified metal-organic framework composite material are used as signal tags for simultaneously detecting multiple components; and the immunosensor platform is prepared from the Au / rGO composite material. The electrochemical immunosensor provided by the invention has the advantages of wide linear range, high sensitivity, good selectivity, greenness, simplicity and the like, provides an effective method for simultaneously detecting two biomarkers of acute myocardial infarction (AMI) in clinical serum, has certain application potential in the aspect of early accurate diagnosis of AMI, and has a bright application prospect; and the method has enlightening significance on detection of other related biomarkers.

Description

technical field [0001] The invention belongs to the technical field of metal-organic framework materials and biosensors, and in particular relates to an electrochemical immunosensor of an electroactive substance-modified MOF composite material and its preparation and application. Background technique [0002] Cardiovascular disease (CVD) accounts for one-third of the global death toll each year, posing a serious threat to human health. Among them, acute myocardial infarction (AMI) has high morbidity and mortality, especially in the early stage of the onset of the highest risk of death. Therefore, providing direct and reliable diagnostic information in the early stages of AMI is crucial for timely treatment of the disease. Currently, clinical assessment, ECG, and monitoring of AMI biomarker levels are routine diagnostic methods for AMI. Among them, the detection of AMI markers is a cost-effective and widely used method for evaluating myocardial dysfunction and the severity ...

AI Technical Summary

Problems solved by technology

However, it is worth noting that electrochemical immunosensors for the simultaneous determination of multi-component markers still have some deficiencies.

First, most of the reported electrochemical methods require expensive and complex signaling molecule labeling processes, which limits the development of multicomponent electrochemical biosensors; second, some sensors rely on enzyme-assisted reactions to amplify the signal, which increases analysis time and Cost; In addition, some electrochemical immunosensors have low loads of antibodies and signal labels, which is not conducive to the formation and amplification of signals, resulting in low sensor sensitivity

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