SARS-CoV-2 detection kit based on sandwich method

Abstract

The invention relates to the field of biological medicine, in particular to an SARS-CoV-2 detection kit based on a sandwich method. The kit comprises (a) a solid phase conjugate. The solid-phase conjugate consists of a solid-phase carrier as well as an antigen and an antibody which coat the solid-phase carrier. The antibody is a monoclonal antibody for resisting novel coronavirus nucleocapsid N protein. The antigen comprises an S1 subunit, an S2 subunit, an RBD, an N protein and an E protein of the SAR-CoV-2 Spike protein. The kit further comprises (b) an antigen and an antibody marked with a chemiluminescent marker. The antibody is a monoclonal antibody to the novel coronavirus nucleocapsid N protein, but is bound to a different epitope from the antibody in (a). The antigen comprises an S1 subunit, an S2 subunit, an RBD, an N protein and an E protein of the SAR-CoV-2 Spike protein.

Description

technical field

[0001] The invention relates to the field of biomedicine, in particular to a SARS-CoV-2 detection kit based on a sandwich method. Background technique

[0002] The new type of coronavirus (SARS-CoV-2) belongs to the genus β coronavirus, the particles are round or oval, with a diameter of 60nm-140nm, and compared with SARS, it has a higher infectivity. The new coronavirus has a strong ability to spread from person to person, and the crowd is generally susceptible. Patients are the most important source of infection. Therefore, early detection, early isolation, and early treatment of asymptomatic infections, suspected and confirmed cases are still the next epidemic situation. The main line of prevention and control. How to accurately detect the virus, quickly identify patients with new crown infection, and reduce cross-infection; and how to effectively monitor the disease process and antibody production will be the focus of the battle against the epidemic. Ba...

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