Method for medium-temperature cultivation of pleurotus tuber-regium sclerotia
The technology of tiger milk mushroom and tiger milk mushroom is applied in the field of sclerotia of tiger milk mushroom cultivation at medium temperature, can solve the problems of uncontrolled sclerotium formation, low artificial cultivation yield, scarcity of wild resources, etc., and achieves a simple domestication cultivation method. , high edible and medicinal value, the effect of reducing cultivation conditions
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Embodiment 1
[0039] The preparation and the propagation method of the original seed culture material of tiger milk mushroom comprise the following steps:
[0040] Step 1. The main material of the original seed compost is mixed with poplar branches, wheat, wheat bran, etc., and the auxiliary materials include gypsum and lime (recipe: 20~35 poplar branches 40%, wheat 40%, wheat bran 18% , lime gypsum each 1%);
[0041] Step 2. Soak the poplar branches and wheat in water 24 hours in advance, and mix the culture materials according to the formula, with a water content of 60%;
[0042] Step 3, using a 500ml tissue culture bottle as a cultivation container;
[0043] Step 4, filling the tissue culture bottle with culture material;
[0044] Step 5. Sterilize by using high-pressure steam at a temperature of 121° C. for 100-120 minutes;
[0045] Step 6. After the sterilization is completed, take it out and cool it down;
[0046] Step 7, cooling, inoculation, and cooling the cultivation medium to...
Embodiment 2
[0050] The manufacturing method of tiger milk mushroom sclerotia culture bag comprises the following steps:
[0051] Step 1. Cultivation material The main material is a mixture of sawdust, corncobs, cottonseed husks, wheat bran, etc. The ingredients include gypsum and lime (recipe: 35% wood chips, 35% corncobs, 10% cottonseed husks, 18% wheat bran , lime gypsum each 1%);
[0052] Step 2. According to the formula, mix each compost well, and the water content is 60%;
[0053] Step 3, with 15cm * The 35cm fungus bag is the material for the cultivation bag, and the material in each bag is compacted to one-half;
[0054]Step 4, install the bacteria bag and put on the collar, put the collar to the mouth of the bag, and the middle is empty;
[0055] Step 5. Sterilize by using high-pressure steam at a temperature of 121°C for 100-120 minutes;
[0056] Step 6. After the sterilization is completed, take it out and cool it down;
[0057] Step 7, cooling, inoculation, inoculation whe...
Embodiment 3
[0061] The production method of the tiger milk mushroom sclerotia nutrition package comprises the following steps:
[0062] Step 1. Cultivation materials are mainly made of wood chips, corncobs, cottonseed hulls, wheat bran, etc. The ingredients include gypsum and lime (recipe: 45% wood chips, 35% corn cobs, 18% wheat bran, 1 lime gypsum each) %);
[0063] Step 2. According to the formula, mix each compost well, and the water content is 60%;
[0064] Step 3, with 18cm * The 35cm bacteria bag is a cultivation bag;
[0065] Step 4, fill the bag with material, that is, each bag is filled with material.
[0066] Step 5. Sterilize by using high-pressure steam at a temperature of 121°C for 100-120 minutes;
[0067] Step 6. After the sterilization is completed, take it out and cool it down;
[0068] Step 7, cooling, inoculation, inoculation when the cultivation medium is cooled to about 25°C, the bacteria used for inoculation are secondary original species, and the inoculum amou...
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