Lactobacillus plantarum for preventing and/or treating periodontitis, its culture and its preparation and application

A technology of Lactobacillus plantarum and culture, applied in the field of microorganisms, can solve problems such as increasing adverse drug reactions, destroying oral micro-ecological environment, and microbial drug resistance

Active Publication Date: 2022-06-17
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, clinical investigations have found that long-term use of metronidazole, minol hydrochloride and other drugs can cause stomach discomfort in some patients and increase the probability of adverse drug reactions, leading to the emergence of drug resistance in the microorganisms in the human body and destroying the normal oral cavity. micro-ecological environment

Method used

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  • Lactobacillus plantarum for preventing and/or treating periodontitis, its culture and its preparation and application
  • Lactobacillus plantarum for preventing and/or treating periodontitis, its culture and its preparation and application
  • Lactobacillus plantarum for preventing and/or treating periodontitis, its culture and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0075] The preparation method of the bacterial suspension involved in the following examples is as follows:

[0076] Clostridium nucleatum bacterial suspension: Inoculate the Clostridium nucleatum bacteria into the BHI medium with an inoculum amount of 2% of the total volume of the BHI medium, and adjust the concentration of the bacterial solution with the BHI medium after anaerobic cultivation at 37°C for 48 hours. 1×10 9 CFU / mL.

[0077] Porphyromonas gingivalis bacterial suspension: inoculate the Porphyromonas gingivalis cells into the BHI medium at an inoculum amount of 2% of the total volume of the BHI medium, and then use the BHI medium after anaerobic culture at 37°C for 48 hours. Adjust the bacterial concentration to 1 × 10 9 CFU / mL.

[0078] Prevotella intermedia bacteria suspension: Inoculate the Prevotella intermedia in the BHI medium with an inoculum amount of 2% of the total volume of the BHI medium, and adjust the concentration of the bacterial solution with t...

Embodiment 1

[0083] Example 1 Screening of Lactobacillus plantarum and identification of bacterial species

[0084] 1. Filter

[0085] The kimchi water from Chongqing was taken as the sample. After the sample was pretreated, it was stored in a -80°C refrigerator in 20% glycerol. After taking out and thawing, the sample was mixed well, and 0.5mL of the sample was added to 4.5mL of 9g / L physiological solution. Gradient dilution was performed with saline, and 100 μL of a suitable gradient dilution solution was selected and spread on MRS medium containing 20 g / L agar, and cultured upside down at 37°C for 48 hours. Pick several typical colonies of Lactobacillus plantarum with an inoculating loop, streak and purify three times on MRS medium containing 20g / L agar, pick several purified single colonies and transfer them to MRS medium for enrichment, 30% glycerol Preservation, the strain CCFM1137 and strains 1-14 screened by the same method in the same period were obtained respectively; among them...

Embodiment 2

[0088] The influence of embodiment 2 Lactobacillus plantarum on the amount of mixed bacteria biofilm

[0089] The experiment was divided into two groups, Lactobacillus plantarum-mediated group and blank control group, and each group was repeated 6 times;

[0090] Wherein, the Lactobacillus plantarum mediation group is processed according to the following method:

[0091] (1) The bacterial cells of Lactobacillus plantarum CCFM1137, Lactobacillus plantarum CCFM8724 and Lactobacillus plantarum 1-14 were inoculated into the MRS medium at an inoculum amount of 2% of the total volume of the MRS medium, and cultured at 37°C for 24 hours to obtain The culture solution was centrifuged at 8000 r / min and 4 °C for 5 min, respectively, to obtain the supernatant, and the supernatant was filtered through a 0.22 μm sterile filter to obtain a bacterial concentration of 10. 9 CFU / mL supernatant of Lactobacillus plantarum CCFM1137, Lactobacillus plantarum CCFM8724, Lactobacillus plantarum 1-14;...

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Abstract

The invention relates to the technical field of microbes, and discloses Lactobacillus plantarum for preventing and / or treating periodontitis, its culture and its preparation and application. The Lactobacillus plantarum, named Lactobacillus plantarum (Lactobacillus plantarum) CCFM1137, was preserved on August 1, 2020 at the Guangdong Provincial Microbial Culture Collection Center on the 5th Floor, Building 59, Compound No. 100, Xianlie Middle Road, Guangzhou City, and the preservation number is GDMCC No: 61114. The plantarum lactobacillus can inhibit the formation of Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum mixed bacteria biofilm; it can reduce the content of inflammatory factors in the periodontitis cell model; it can make the periodontitis cell model The expression level of the transmembrane transport protein is increased; it can be used for preventing and / or treating oral diseases and inhibiting Prevotella intermedium, Porphyromonas gingivalis and / or Fusobacterium nucleatum.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to Lactobacillus plantarum for preventing and / or treating periodontitis, a culture thereof, and preparation and application thereof. Background technique [0002] Periodontitis is an infectious inflammatory disease that occurs in the supporting tissues of the oral cavity. Periodontitis is usually accompanied by symptoms such as bad breath, swollen and bleeding gums, loose teeth, and masticatory dysfunction. It is also the leading cause of tooth loss in adults. More and more studies have shown that periodontitis pathogens such as Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella intermedia are closely related to a variety of systemic diseases, such as diabetes, Alzheimer's disease, and colon cancer. [0003] Periodontitis is a multifactorial disease. In addition to plaque microorganisms and their products, which are essential initiating factors, the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61K36/539A61P1/02A61P31/04A61K8/99A61K8/9789A61Q11/00A61Q17/00A23L33/105A23L33/135C12R1/25
CPCC12N1/20A61K35/747A61K36/355A61K36/539A61P1/02A61P31/04A61K8/99A61K8/9789A61Q11/00A61Q17/005A23L33/105A23L33/135A23V2002/00A23V2400/169A61K2300/00A23V2200/312A23V2200/3204
Inventor 郑彦懿胡明华张秋香徐晚晴陆文伟
Owner INFINITUS (CHINA) CO LTD
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