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Facultative nutritional ammonia oxidizing bacteria and application thereof

A strain of Rhodococcus, applied in the field of environmental microorganisms, to achieve the effect of avoiding carbon source input, increasing processing costs, and enhancing environmental tolerance

Active Publication Date: 2021-06-22
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few reports on facultative trophic nitrifying bacteria. The data show that only some strains of Nitrobacter winogradskyi can grow on some organic media, but these microorganisms are non-spore-free Gram-negative bacteria.

Method used

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  • Facultative nutritional ammonia oxidizing bacteria and application thereof
  • Facultative nutritional ammonia oxidizing bacteria and application thereof
  • Facultative nutritional ammonia oxidizing bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Screening and Identification of Bacterial Strains

[0034] 1. Prepare the medium as follows:

[0035] (1) Nitrification (autotrophic) medium: KHCO 3 1g / L, KH 2 PO 4 0.5g / L, CaCl 2 2H 2 O0.3g / L, MgSO 4 ·7H 2O 1g / L, (NH 4 ) 2 SO 4 2g / L, CaCO 3 2g / L. In addition, 2 mL of trace elements I and 2 mL of trace elements II were added per liter. The final pH of the medium was between 7.0 and 7.5. Increase agarose 12g / L, namely solid nitrifying (autotrophic) medium.

[0036] Trace element solution Ⅰ: EDTA 20.71g / L and FeSO 4 ·7H 2 O 9.15g / L.

[0037] Trace element solution Ⅱ: ZnSO 4 ·7H 2 O 430mg / L, MnCl 2 4H 2 O 990mg / L, H 3 BO 3 14mg / L, CoCl 2 ·6H 2 O 240mg / L, CuSO 4 ·5H 2 O 250mg / L, NiCl 2 ·6H 2 O190mg / L, (NH 4 ) 6 Mo 7 o 24 4H 2 O550mg / L.

[0038] (2) Nitrification (heterotrophic) medium: (NH 4 ) 2 SO 4 2.0g / L, glucose 9.09g / L, sodium acetate 6.20g / L, sodium succinate 6.13g / L, sodium citrate 7.42g / L, sucrose 4.32g / L, Vickers...

Embodiment 2

[0075] Example 2: Synergistic treatment of high-concentration wastewater COD and ammonia nitrogen by nitrifying bacteria CNOx

[0076] With the fermented broth of 6L nitrifying bacteria CNOx (viable bacteria concentration ≥ 10 9 CFU / mL) were collected by low-temperature centrifugation, and then divided into two parts with equal mass in the ultra-clean workbench, one of which was sterilized at 115°C for 30 minutes as a control treatment, and the other was temporarily stored at 4°C. After the sterilization is finished, inoculate two parts of bacteria into 8L plexiglass tanks filled with 6L of high-concentration organic wastewater (COD is 15200mg / L, ammonia nitrogen content is 1640mg / L), stir well and then aerate (3L / min) treatment, using sodium carbonate to replenish alkalinity every day, maintaining the pH of the solution between 7.5 and 8.0, and replenishing water according to the initial liquid level, and taking samples to measure COD, ammonia nitrogen, nitrite nitrogen, nit...

Embodiment 3

[0080] Example 3: Application of nitrifying bacteria CNOx in short-range nitrification and denitrification denitrification reactor

[0081] Short-range nitrification and denitrification denitrification technology refers to controlling the nitrification reaction to NH 4 + →NO 2 - stage, to avoid or reduce the occurrence of NO 2 - →NO 3 - conversion, directly to NO 2 - →N 2 Reaction. Compared with the traditional nitrification and denitrification technology, short-cut nitrification and denitrification has the following advantages: the aerobic stage can save 25% of the oxygen consumption; the anoxic stage can reduce the carbon source consumption by 40%. At the same time, due to NO 2 - →N 2 The reaction rate is NO 3 - →N 2 1.5 to 2 times of that, so the denitrification residence time can be greatly shortened. In addition, short-cut nitrification and denitrification can effectively reduce sludge production. Through the screening test of the strains in Example 1, i...

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Abstract

The invention belongs to the field of environmental microorganisms, and particularly relates to a facultative nutritional ammonia oxidizing bacterium and application thereof. According to the specific technical scheme, the rhodococcus is preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 17, 2020, the strain name is rhodococcus sp. (CNOX), and the preservation number is CGMCC No.21393. The invention provides a brand-new nitrifying bacterium CNO < x > which is a new species. Different from previous studies, the nitrifying bacteria CNOx are gram-positive and facultative nutritional nitrifying bacteria, have typical characteristics of autotrophic and heterotrophic nitrifying microorganisms, can realize rapid expanding culture through heterotrophic growth, can also perform autotrophic nitrification to avoid carbon source investment and increase treatment cost, and is hardly inhibited by high-concentration ammonia nitrogen and organic matters, has extremely strong environmental endurance capacity, and is an ideal biological material for artificially cultivating and preparing a nitrifying bacterium agent.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a facultative trophic ammonia oxidizing bacterium and its application. Background technique [0002] Chemical industry, leather industry, coking industry, pharmaceutical industry, breeding industry and domestic garbage all produce a large amount of high-ammonia nitrogen wastewater, which will cause eutrophication of the water body after being discharged into the water body, seriously affecting the growth and reproduction of aquatic organisms. At present, the denitrification treatment in sewage is mainly carried out through physical, chemical and biological methods, but the cost of physical method (membrane treatment) is relatively high, and chemical method is easy to cause secondary pollution of water body, while biological method is environmentally friendly, has no residue, is safe and reliable And other characteristics, so become the first choice. [000...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/30C02F3/34C12R1/01
CPCC02F3/301C02F3/302C02F3/34C02F2209/06C02F2209/02C02F2003/003C02F2305/06
Inventor 李东曹沁陈意超赖生平聂明建杨洪伟廖杰高雪莉
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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