Method for extracting isothiocyanate high-purity product from brassicaceous vegetable seeds

Abstract

The invention discloses a method extracting an isothiocyanate high-purity product from brassicaceous vegetable seeds. According to the method, fresh mature brassicaceous vegetable seeds which containrice resources and have a high glucosinolate content are utilized as a raw material. The method comprises the steps: firstly, adding water into the raw material, grinding the raw material into slurryand performing fermentation treatment; then sequentially utilizing advanced extracting technologies of ethyl acetate extraction, nanofilm preliminary purification, polyamide resin purification, HP2MGLresin secondary purification, crystallization and the like to take technical breakthrough in factory scale production of the isothiocyanate high-purity product and obtain high-purity isothiocyanate extract. Not only is the excellent raw material is provided for researching pharmacological action and drug efficacy and developing a functional terminal production, but also safe, convenient, reasonable and economical high-valued utilization of the brassicaceous vegetable seed resources is really achieved, and a green chemical production idea and a cyclic economy strategy are fully expressed.

Description

technical field [0001] The invention relates to a method for extracting high-purity isothiocyanate from cruciferous vegetable seeds, especially a method of firstly adding water to grind the raw material into a slurry and performing fermentation treatment, followed by ethyl acetate extraction and nano-membrane preliminary Purification, polyamide resin purification, HP2MGL resin re-purification, crystallization and other extraction processes to prepare high-purity isothiocyanate. Background technique [0002] Glucosinolates are an important class of secondary metabolites in plants. Isothiocyanate is the enzymatic hydrolysis product of glucosinolate, which is widely and abundantly present in cruciferous vegetable plants, under the action of its myrosinase. Studies have shown that the content of glucosinolate and myrosinase in mature mustard, broccoli and other vegetable seeds is high, but in normal vegetable plant tissues, glucosinolate and myrosinase exist independently of ea...

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Problems solved by technology

[0003] At present, the extraction methods of isothiocyanate mainly include pressing method, steam distillation method, ultrasonic or microwave-assisted extraction method, enzyme-assisted extraction method, etc., and the separation and purification methods mainly include solvent extraction method, supercritical fluid extraction method, large-scale Pore ​​resin adsorption method, low-pressure silica gel column chromatography, high-speed countercurrent chromatography, etc., but these methods are mostly limited to the experimental research on the effect of a single technology and process on the extraction or separation and purification of isothiocyanates. Compared with its material composition, the content of glucosinolates in organs (including seeds) is generally less than 1%, and there are significant differences due to different vegetable varieties, growth environments, planting techniques and picking parts, making different extraction and preparation In the thiocyanate process, there are technical bottlenecks such as low yield, low purity, and large consumption of processing raw materials. For example, the Chinese published patent application CN1405316A, the crude isothiocyanate extract prepared by extraction cannot meet the requirements of the development of functional end products. Quality requirements, as well as the general public's market demand for isothiocyanate functional end products, establishing a method suitable for factory-scale production of high-purity isothiocyanate has become an urgent task in this field