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Method for increasing contents of genistin and genistein in flemingia macrophylla by using calcium

A technology of genistin and genistein, which is applied in the field of medicinal plant breeding, can solve the problems of low content and affecting the utilization rate of Echinacea chinensis, and achieve the effects of increasing content, improving utilization rate, and promoting rooting

Active Publication Date: 2021-06-25
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the content of genistin and genistein in Echinacea macrophylla is low, which affects the utilization rate of Echinacea macrophylla

Method used

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  • Method for increasing contents of genistin and genistein in flemingia macrophylla by using calcium
  • Method for increasing contents of genistin and genistein in flemingia macrophylla by using calcium
  • Method for increasing contents of genistin and genistein in flemingia macrophylla by using calcium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The method of using calcium to increase the content of genistin and genistein in Echinacea chinensis, placing the tissue cultured seedlings of Echinacea chinensis in the first medium for cultivation, wherein the first medium includes 1 / 2MS, calcium chloride aqueous solution.

[0025] The concentration of calcium chloride aqueous solution is 4.40g / 500mL.

[0026] The consumption of calcium chloride aqueous solution is 25mL.

[0027] The first medium also includes 0.3mg·L -1 NAA+20.0g·L －1 Sucrose+4.0g·L －1 Agar powder+0.5g·L －1 Activated carbon.

[0028] The culture conditions are: the temperature is 25-28°C, the light time is 10h / d, 60d.

Embodiment 2

[0030] The method of using calcium to increase the content of genistin and genistein in Echinacea chinensis, placing the tissue cultured seedlings of Echinacea chinensis in the first medium for cultivation, wherein the first medium includes 1 / 2MS, calcium chloride aqueous solution.

[0031] The concentration of calcium chloride aqueous solution is 4.40g / 500mL.

[0032] The consumption of calcium chloride aqueous solution is 50mL.

[0033] The first medium also includes 0.3mg·L -1 NAA+20.0g·L －1 Sucrose+4.0g·L －1 Agar powder+0.5g·L －1 Activated carbon.

[0034] The culture conditions are: the temperature is 25-28°C, the light time is 10h / d, 60d.

Embodiment 3

[0036] The method of using calcium to increase the content of genistin and genistein in Echinacea chinensis, placing the tissue cultured seedlings of Echinacea chinensis in the first medium for cultivation, wherein the first medium includes 1 / 2MS, calcium chloride aqueous solution.

[0037] The concentration of calcium chloride aqueous solution is 4.40g / 500mL.

[0038] The consumption of calcium chloride aqueous solution is 75mL.

[0039] The first medium also includes 0.3mg·L -1 NAA+20.0g·L －1 Sucrose+4.0g·L －1 Agar powder+0.5g·L －1 Activated carbon.

[0040] The culture conditions are: the temperature is 25-28°C, the light time is 10h / d, 60d.

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Abstract

The invention discloses a method for increasing the contents of genistin and genistein in flemingia macrophylla by using calcium. Tissue culture seedlings of flemingia macrophylla are placed in a first culture medium for culture, wherein the first culture medium comprises 1 / 2MS and a calcium chloride aqueous solution. The concentration of the calcium chloride aqueous solution is 4.40 g / 500 mL, and the dosage is 5-150 mL. The dosage of the calcium chloride aqueous solution is 25-100 mL. The first culture medium further comprises 0.3 mg / L of NAA, 20.0 g / L of sucrose, 4.0 g / L of agar powder and 0.5 g / L of activated carbon. According to the method, accumulation of active ingredients of flemingia macrophylla is adjusted by adding exogenous calcium ions, and the contents of genistin and genistein in tissue culture seedlings of flemingia macrophylla can be effectively increased.

Description

technical field [0001] The invention relates to the technical field of medicinal plant breeding. More specifically, the present invention relates to a method for increasing the content of genistin and genistein in Rhizoma chinensis by using calcium. Background technique [0002] Daye Qianjinba (Flemingia macrophylla (Willd.) Merr., also known as Big Pigtail, Qianjinli, Qianjinhong, Hongyaotou and White Horse Shit, etc., is a folk medicine commonly used by ethnic minorities in Southwest China. It is often used in Zhuang and Hani. , Yao, Dai and other national medicines have high nutritional and medicinal value, and are both medicinal and edible plants. It is sweet, slightly warm, and flat, and has the effects of anti-inflammatory, analgesic, hemostatic, and stasis. It is one of the mainstream varieties of Qianjinba medicinal materials currently on the market, and it is the main raw material of Chinese patent medicines such as Fukeqianjin Tablets, Jinji Capsules, Zhuangyao Ji...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 韦坤华梁莹李林轩蔡锦源韦范秦双双林杨李翠
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS