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Lipid nanoemulsion with effect of removing reactive oxygen free radicals in cells, and preparation method thereof

A lipid nanotechnology, cell removal technology, applied in food ingredients as emulsifier, application, food homogenization and other directions, can solve problems such as single chemical substances, achieve controllable particle size, good physical and chemical stability, and biological safety. significant effect

Pending Publication Date: 2021-06-25
FISHERIES RES INST OF FUJIAN +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, domestic and foreign research on echinacea mainly focuses on single chemical substances, such as echinacea polysaccharides, separation and purification of polypeptides, immune activity and its products (including instant sea urchins, oral liquids, skin care products), etc., such as patents CN201510574046.9, CN201310248186.8, CN201010525618.1, CN200810011855, but there is no report on the preparation of sea urchin yellow lipid, fat-soluble trace bioactive substances, microfluidized nanoemulsion and its effect on removing active oxygen free radicals in cells

Method used

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  • Lipid nanoemulsion with effect of removing reactive oxygen free radicals in cells, and preparation method thereof
  • Lipid nanoemulsion with effect of removing reactive oxygen free radicals in cells, and preparation method thereof
  • Lipid nanoemulsion with effect of removing reactive oxygen free radicals in cells, and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation method of embodiment 1 sea urchin yellow lipid nanoemulsion

[0035] (1) Preparation of sea urchin yellow lipid

[0036] 1. Pretreatment

[0037] Open the sea urchin shell, take out the sea urchin yellow, rinse with 1% salt water, drain the water, and vacuum freeze-dry.

[0038] 2. Ultrasonic-assisted organic solvent extraction

[0039] (1) Ethyl acetate system (EAC) is used. The ratio of solid to liquid is 1:20 (w / v, g / mL), protected from light, 25°C, ultrasonic frequency 100Hz, ultrasonic for 30 min, and the temperature of the water bath is adjusted to 25°C every 10 minutes. Stir at room temperature for 20 minutes, centrifuge, filter the extract through a membrane, protect from light, concentrate under reduced pressure at 35°C under nitrogen flow, and dry to constant weight. The yield is 20.6%.

[0040] (2) Ethanol system (ETH) is used. The ratio of solid to liquid is 1:20 (w / v, g / mL), protected from light, 25 °C, ultrasonic frequency 100 Hz, ultras...

Embodiment 2

[0055] The preparation method of embodiment 2 sea urchin yellow lipid alcohol extract

[0056] Select ethanol as the extractant, and its extract is oil alcohol extract, and the specific steps are as follows:

[0057] Weigh 4.0 g of sea urchin yellow lipid prepared in different solvent systems into a glass centrifuge tube, add 5 mL of absolute ethanol, vortex and shake at room temperature for 5 min in the dark, and centrifuge, transfer the upper alcohol extract to a brown glass sample bottle , repeated the extraction 3 times, and combined the upper ethanol extracts to obtain 0.2 g / mL echinacea lipid alcohol extract.

Embodiment 3

[0058] Example 3 Cell proliferation-toxicity evaluation

[0059] The human liver cancer cell line HepG-2 was used to evaluate the cell proliferation-toxicity of quercetin, echinacein lipid alcohol extract, emulsion and nanoemulsion. Quercetin was used as the standard antioxidant compound and quercetin was dissolved using DMSO. Quercetin, echinacea lipid alcohol extract, echinacea lipid emulsion and echinacea lipid nanoemulsion were diluted with MEM-α basal medium containing 1% antibacterial-antifungal agent.

[0060] Use MEM-α complete medium containing 10% fetal bovine serum and 1% antibacterial-antifungal agent at a final concentration of 37°C in 5% CO 2 Culture HepG-2 cells. HepG-2 cells were divided into 1×10 5 cells / mL, 100 μL / well, were inoculated into a transparent bottom 96-well plate, with 6 parallel wells in each group, and cultured for 24 h adherently. The original medium was discarded, and 100 μL / well of the diluted sample (experimental group) and MEM-α basal m...

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Abstract

The invention relates to a preparation method of a lipid nanoemulsion with an effect of removing reactive oxygen free radicals in cells. The preparation method comprises the following steps: preparing an emulsifier, preparing a sea urchin yellow lipid emulsion, and preparing the sea urchin yellow lipid nanoemulsion. The preparation method of the sea urchin yellow lipid comprises the steps of vacuum freeze drying of sea urchin yellow, ultrasonic-assisted organic solvent extraction, centrifugal concentration and the like. Compared with a traditional chemical model or an intracellular anti-oxidation model of a grease alcohol extract, the sea urchin yellow lipid nanoemulsion and the intracellular anti-oxidation model of the sea urchin yellow lipid nanoemulsion can more accurately reflect the effect of removing reactive oxygen free radicals of grease in vivo; The preparation method of the sea urchin yellow lipid nanoemulsion is green, safe and simple to operate, chemical crosslinking and construction are not needed, and the prepared sea urchin yellow lipid nanoemulsion is controllable in nanoparticle size, good in physical and chemical stability and biological safety and remarkable in effect of removing reactive oxygen free radicals in cells.

Description

technical field [0001] The invention belongs to the technical field of food nutrition and nanometer materials, and in particular relates to a lipid nanoemulsion capable of removing intracellular active oxygen free radicals and a preparation method thereof. Background technique [0002] Lipid is one of the important nutritional elements for the survival of organisms. In addition to providing the required energy for the organism, it is closely related to the occurrence and development of many diseases, and plays a key role in maintaining the homeostasis and health of the organism. [0003] Sea urchin yellow is the gonad of sea urchins, and it is a healthy ingredient that is highly sought after by gourmet gourmets. Its lipids account for about 10-30% of the total weight (dry basis), including fatty acids, glycerides, phospholipids, sterol lipids, prenol esters, glycolipids and fat-soluble trace bioactive substances, etc. It has a fresh, mellow and sweet taste, and is beneficia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/115A23L29/10
CPCA23L33/115A23L29/10A23V2002/00A23V2200/30A23V2200/222A23V2250/5436A23V2300/14A23V2300/48A23V2300/26Y02A50/30
Inventor 潘南刘智禹吴靖娜蔡水淋陈晓婷李毅李颖
Owner FISHERIES RES INST OF FUJIAN
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