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Efficient valine preparation method

A high-efficiency technology for valine, applied in the field of high-efficiency valine preparation, can solve the problems of low conversion rate of sugar and acid, high content of miscellaneous acids, and long fermentation cycle, so as to improve fermentation efficiency, reduce the content of miscellaneous acids, The effect of easy operation

Active Publication Date: 2021-06-25
ANHUI HUAHENG BIOTECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has defects such as long fermentation period, low sugar-acid conversion rate (30-40%), and high heteroacid content in the product.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Take the Sval065 strain preserved in a 1mL glycerol tube (preservation number is CGMCC NO.19458), inoculate it into 50mL of LB medium, culture it on a shaker at 37°C and 210r / min, and adjust the pH to 6.2-6.5 , at the end of the cultivation until the OD value is 2-3, the first-grade seed liquid is obtained;

[0049] (2) Inoculate 2 mL of primary seed solution into 100 mL of synthetic medium, culture on a shaker at 37°C and 210 r / min, adjust the pH to 6.4-6.6, and cultivate until the OD value is 3-4 to obtain secondary seeds liquid;

[0050] (3) In a flame-protected environment, inoculate the secondary seed liquid into the fermentation medium at an inoculum size of 2.5%, cultivate at 35°C for 30 hours, and cultivate at 43°C until the amount of residual sugar is less than 2g / L, wherein the ambient pressure 0.03MPa, the fermentation speed is 200r / min, the air flow rate is 100L / h, and the fermentation pH is adjusted to 6.9-7.0.

[0051] According to statistics, the ferm...

Embodiment 2

[0058] (1) Take the Sval031 strain (preservation number: CGMCC No.19456) preserved in a 1 mL glycerol tube, inoculate it into 50 mL of LB medium, culture it on a shaker at 37°C and 210 r / min, pH 6.2-6.5, and culture When the OD value is 2-3, the first-grade seed solution is obtained;

[0059] (2) Inoculate 2mL of the primary seed solution into 100mL of synthetic medium, and culture it on a shaking table under the conditions of 37°C, 210r / min, and pH6.4-6.6 until the OD value is 3-4 to obtain the secondary seed solution ;

[0060] (3) Under the flame protection environment, inoculate the secondary seed liquid into the fermentation medium with an inoculum size of 2.5%, cultivate it to a stable growth period at 35°C, and cultivate it until the residual sugar amount is less than 2g / L at 43°C, wherein , the ambient pressure is 0.03MPa, the fermentation speed is 200r / min, the air flow rate is 100L / h, and the fermentation pH is 6.9-7.0.

[0061] According to the statistics, the fer...

Embodiment 3

[0068] (1) Take the Sval049 strain preserved in a 1mL glycerol tube (preservation number: CGMCC No.19457), inoculate it into 50mL of LB medium, and culture it on a shaker at 37°C, 210r / min, pH6.2-6.5 until OD The value is 2-3, and the first-grade seed liquid is obtained;

[0069] (2) Take 2 mL of the primary seed liquid and inoculate it into 100 mL of synthetic medium, culture it on a shaker at 37°C, 210 r / min, and pH 6.4-6.6 until the OD value is 3-4, and obtain the secondary seed liquid;

[0070] (3) Under the flame protection environment, inoculate the secondary seed liquid into the fermentation medium at an inoculum size of 2.5%, cultivate it to a stable growth phase at 35°C, and cultivate it until the amount of residual sugar is less than 2g / L at 43°C, wherein , the ambient pressure is 0.03MPa, the fermentation speed is 200r / min, the air flow rate is 100L / h, and the fermentation pH is 6.9-7.0.

[0071] According to statistics, the fermentation time is 52 hours. After the...

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PUM

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Abstract

The invention relates to an efficient valine preparation method, which comprises the following steps: (1) inoculating valine producing bacteria into an LB culture medium according to the inoculum size of 1-10%, and culturing a culture system of the valine producing bacteria under the conditions that the speed is 180-220 r / min, the temperature is 35-37 DEG C and the pH value is 6-7 until the OD value is 3-4 to obtain a primary seed solution; (2) transferring the primary seed solution into a synthetic culture medium according to the inoculum size of 1-10%, placing the synthetic culture medium under the conditions of 180-220 r / min, 35-37 DEG C and pH of 6-7, and culturing until the OD value is 3-4 to obtain a secondary seed solution; and (3) inoculating the secondary seed solution into a fermentation culture medium according to the inoculum size of 1-10%, carrying out fermentation culture at 30-37 DEG C under the pH value of 6-7 until a stable growth period, and continuously carrying out fermentation culture under the conditions of 38-45 DEG C under the pH value of 6-7 until the residual sugar content is not higher than 2g / L, thereby obtaining the valine fermentation liquor. According to the method, the saccharic acid productivity is improved while the valine conversion rate is ensured, and the content of heteroacid in the product can be reduced, so that the subsequent purification difficulty is reduced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a high-efficiency valine preparation method. Background technique [0002] Valine is an essential amino acid and nutrient for the body. It is widely used in food additives, feed additives, nutritional supplements, flavoring agents, cosmetic additives, and drugs (such as antibiotics, herbicides, etc.) in the fields of food, medicine, cosmetics, and feed. Preparation of precursors, etc., the market prospect is very broad. [0003] CN110607268A discloses a valine fermenting genetically engineered bacterium and a fermentation method thereof. The fermentation period of the method is 40 hours, and the valine output is 80g / L. However, this method has defects such as long fermentation period, low sugar-acid conversion rate (30-40%), and high heteroacid content in the product. [0004] For this reason, it is necessary to research and develop a method for preparing valine with high conversio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/08C12N1/20C12R1/19
CPCC12P13/08C12N1/20
Inventor 马祥亮刘树蓬刘磊张大伟余军
Owner ANHUI HUAHENG BIOTECH