Fingerprint spectrum construction method and application of fructus evodiae and asarum preparation

A fingerprint and construction method technology, which is applied in the field of fingerprint construction of evodia rutaecarpa and asarum preparations, to achieve the effects of simple operation, product quality and clinical drug safety, and rapid detection

Active Publication Date: 2021-06-25
杭州益瑞医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the content determination method for specific components has been established, but to reflect its quality as a whole, it is still necessary to use the fingerprint method

Method used

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  • Fingerprint spectrum construction method and application of fructus evodiae and asarum preparation
  • Fingerprint spectrum construction method and application of fructus evodiae and asarum preparation

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preparation example Construction

[0023] (1) Preparation of the test solution: accurately weigh an appropriate amount of Evodia rutaecarpa Asarum preparation, and accurately add 20-100 times the amount of methanol-water (w / v), the volume ratio of methanol-water is 8:2-10, Accurately weigh the weight, 30 ~ 50KHz, 100 ~ 300W ultrasonic treatment for 10 ~ 60min, methanol - water to make up the lost weight, filter through a 0.45μm microporous membrane, and take the subsequent filtrate to obtain the test solution (the subsequent filtrate is Analysis of commonly used terms, in order to discard the initial part of the filtrate, take the latter part of the filtrate. The initial part of the filtrate may be absorbed by the filter material, which is not accurate enough);

[0024] (2) Preparation of reference substance solution: Precisely weigh an appropriate amount of evodiamine, evodiamine, and limonin reference substances, dissolve them in methanol and constant volume, and prepare them to contain 50-150ug / ml evodiamine ...

Embodiment 1

[0039] This implementation case shows a fingerprint construction method of Evodia rutaecarpa Asarum preparation according to the following steps:

[0040] 1. Preparation of the test solution

[0041]Accurately weigh 0.5 g of Evodia rutaecarpa Asarum preparation, add 25 mL of methanol-water (volume ratio 8:2), accurately weigh, ultrasonically treat for 30 minutes (250W, 45KHz), and use methanol-water (volume ratio 8:2) to supplement the Lost weight, shake well, filter through a 0.45 μm microporous membrane, and take the subsequent filtrate to obtain the test solution.

[0042] 2. Preparation of reference solution

[0043] Accurately weigh the appropriate amount of evodiamine, evodiamine, and limonin reference substances, dissolve them in methanol and constant volume, and prepare them respectively as controls containing 80ug / ml evodiamine, 50ug / ml evodiamine, and 100ug / ml limonin. product solution.

[0044] 3. Chromatographic conditions

[0045] Chromatographic column: Agile...

Embodiment 2

[0059] This implementation case shows a fingerprint construction method of Evodia rutaecarpa Asarum preparation according to the following steps:

[0060] 1. Preparation of sample solution to be tested

[0061] Accurately weigh 0.5g of Evodia rutaecarpa Asarum preparation (S11), add 50ml of methanol-water (8:2) accurately, accurately weigh the weight, ultrasonically treat for 15min (35KHz, 300W), add methanol-water (8:2) to make up The lost weight was filtered through a 0.45 μm microporous membrane, and the filtrate was taken to obtain the sample solution to be tested.

[0062] 2. Preparation of reference solution

[0063] Accurately weigh the appropriate amount of evodiamine, evodiamine, and limonin reference substances, dissolve them in methanol and make up to volume, and prepare a mixed reference substance containing 50ug / ml evodiamine, 30ug / ml evodiamine, and 150ug / ml limonin solution, namely the reference solution.

[0064] 3. Fingerprint analysis

[0065] Get 30 μ L ...

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Abstract

The invention discloses a fingerprint spectrum construction method and application of a fructus evodiae and asarum preparation. The method is established by adopting a high performance liquid chromatography. The chromatographic conditions are as follows: octadecylsilane chemically bonded silica is used as a filler, acetonitrile is used as a mobile phase A, a phosphoric acid aqueous solution with the mass concentration of 0.02% is used as a mobile phase B, gradient elution is performed, the flow rate is 1.0 ml.min<-1>, the column temperature is 35 DEG C, and the detection wavelength is 215 nm. The method is easy and convenient to operate, high in stability and good in reproducibility, the obtained spectrum has multiple characteristic peaks, the quality of the fructus evodiae and asarum preparation of the product can be comprehensively evaluated by comparing the characteristic spectrum with the characteristic peaks, the product quality can be stabilized, and the safety and effectiveness of clinical medication are ensured.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine pharmacy, and in particular relates to a method for constructing fingerprints of Evodia rutaecarpa Asarum preparation and its application. Background technique [0002] Fingerprint of traditional Chinese medicine refers to the chromatogram or spectrum that can mark the chemical characteristics of some traditional Chinese medicinal materials or preparations after proper treatment and certain analysis methods. Traditional Chinese medicine fingerprint is a comprehensive and quantifiable means of identification. It is based on the systematic research on the chemical components of traditional Chinese medicine and is mainly used to evaluate the authenticity, goodness and stability of the quality of Chinese medicinal materials and semi-finished products of traditional Chinese medicine preparations. "Integrity" and "ambiguity" are its salient features. At present, the quality testing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8686Y02A50/30
Inventor 田景奎陈学钊张晓勇李守信李伟
Owner 杭州益瑞医药科技有限公司
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