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Method for detecting chlorantraniliprole in plants

A technology for chlorantraniliprole and a detection method is applied in the field of detection of chlorantraniliprole in plants, and can solve the problems of low chlorantraniliprole content, poor detection stability, poor chromatographic peak shape and the like , to reduce interference, improve chromatographic peak shape, and improve stability.

Active Publication Date: 2021-06-25
品测(上海)检测科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the relative deviation of the test results after SPE purification treatment is small, but the operation is complicated; the operation of QuEChERS is simpler, and more and more people are studying the QuEChERS method.
[0004] In view of the above-mentioned related technologies, the inventor believes that the content of chlorantraniliprole in crops is low, the chromatographic peak detected after QuEChERS purification treatment is correspondingly low, and the chromatographic peak type Poor, large relative deviation, poor detection stability

Method used

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  • Method for detecting chlorantraniliprole in plants
  • Method for detecting chlorantraniliprole in plants
  • Method for detecting chlorantraniliprole in plants

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Embodiment 1

[0042] Embodiment 1: a kind of detection method of chlorantraniliprole in plants, comprises the following steps:

[0043] S1 extraction: Take 10g (accurate to 0.001g) of oranges to be tested, add them to a 50mL centrifuge tube, add 5g of sodium chloride and magnesium sulfate to form an extraction aid in a ratio of 0.8:1, and then add 10mL containing 0.5wt% The acetonitrile solution of formic acid and 0.5wt% sorbic acid was shaken for 5 minutes, sonicated for 10 minutes, centrifuged once at 8000 rpm for 10 minutes, and the centrifuged supernatant was collected once to obtain an extract.

[0044] S2 Purification: Take 2mL of the extract and add it to a 15mL QuEChERS purification tube, which contains 900mg of MgSO 4 , 150mg of GCB, 150mg of PSA and 25mg of C18, shaken for 5min, centrifuged twice at 8000 rpm for 10min, blown dry with nitrogen in a water bath at 40°C, added 1ml of initial mobile phase to dissolve, passed through a 0.22μm filter membrane, and obtained Purifying flu...

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Abstract

The invention discloses a method for detecting chlorantraniliprole in plants, and relates to the technical field of crop pesticide component detection. The invention discloses a method for detecting chlorantraniliprole in plants, which comprises the following steps: S1, extraction: taking a sample to be detected, adding an extraction aid and an acetonitrile solution of organic acid, uniformly oscillating, centrifuging, and taking supernate to prepare extract liquor; wherein the organic acid is one or two of sorbic acid and formic acid, and the extraction aid is one or two of sodium chloride and magnesium sulfate; S2, purification: adding the extract liquor into a purification tube containing PSA, C18 and GCB, oscillating uniformly, centrifuging, taking supernate, carrying out nitrogen blowing, adding 1mL of an initial mobile phase for dissolving, filtering, and taking filtrate, so as to obtain purified liquor; and S3, detecting on a machine: injecting the purified liquid into a liquid chromatographic column for LC-MS combined detection, eluting, detecting the peak area, and calculating the content of chlorantraniliprole. The method for detecting chlorantraniliprole in plants has the advantage of good stability.

Description

technical field [0001] The present application relates to the field of analysis and detection of crop pesticide components, in particular to a detection method for chlorantraniliprole in plants. Background technique [0002] Chlorantraniliprole is a new type of insecticide that acts on ryanodine receptors (RyRs) by destroying Ca in the cytoplasm of insects. + The stability of the environment causes its muscle regulation to weaken and paralyze until the pest dies. Chlorantraniliprole has reliable and stable control effects at low doses, and is gradually being widely used in pesticides. However, excessive content of chlorantraniliprole in crops will damage human organs. Therefore, all countries have certain requirements for the residue limit of chlorantraniliprole in crops such as citrus. The GB2763-2019 "National Food Safety Standard for Maximum Residue Limits of Pesticides in Food" promulgated by my country sets temporary limit requirements for chlorantraniliprole in food ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/14G01N30/34G01N30/8634Y02A50/30
Inventor 董昕王佳杰吴佳彦康立于源戴辉
Owner 品测(上海)检测科技有限公司
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