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A kind of seed disinfection preparation, its preparation method, use method and application

A technology for disinfecting preparations and seeds, applied in the field of tobacco disease prevention and control, can solve the problems of reducing nicotine, increasing miscellaneous gas, worsening flammability, etc., and achieving the effects of good activity and simple structure

Active Publication Date: 2022-02-11
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the content of sugar in tobacco leaves will decrease, the content of total nitrogen and protein will increase, the irritation will increase, the aroma will be impure, and the miscellaneous gas will increase; the nicotine will decrease, and the flammability will become worse.
This causes serious interference to the strain comparison and screening of editing materials, and in serious cases, it will cause scrapping of key materials and affect the work progress

Method used

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  • A kind of seed disinfection preparation, its preparation method, use method and application
  • A kind of seed disinfection preparation, its preparation method, use method and application
  • A kind of seed disinfection preparation, its preparation method, use method and application

Examples

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Effect test

Embodiment 1

[0052] This example is the preparation method of quinoline alkaloid anti-tobacco mosaic virus active compound, 4-acetyl-7-isopropyl-5-methylquinolin-2(1H)-one of the present invention. The compound uses tobacco stem bark as a raw material, and is obtained through the steps of extracting extract, silica gel column chromatography, and high performance liquid chromatography, specifically:

[0053] A. Extraction of extract: take the stem bark of air-dried gene-edited tobacco as raw material, crush it to 20-40 mesh, use 90-99% ethanol for ultrasonic extraction, and add the extract to the mixture of ethyl acetate and 3% tartaric acid , after the mixed solution was left to stand for stratification, and then Na 2 CO 3 The solution adjusted the pH value of the aqueous layer to 9.0, extracted again with ethyl acetate, filtered, and concentrated under reduced pressure to obtain an extract;

[0054] The gene-edited tobacco is the tobacco in which the NtTOM3 gene is knocked out;

[0055...

Embodiment 2

[0058] This example is a method for identifying the structure of 4-acetyl-7-isopropyl-5-methylquinolin-2(1H)-one of the present invention. The structure of the compound was identified by nuclear magnetic resonance, mass spectrometry, infrared, ultraviolet and other spectral data, specifically:

[0059] The compound of the present invention is a brown jelly;

[0060] The ultraviolet absorption spectrum shows that it has the maximum absorption at 230 and 336nm, which proves that there is an aromatic ring structure in the compound;

[0061] Infrared spectrum (potassium bromide tablet) shows that there are amino groups (3278cm -1 ), carbonyl (1680, 1654cm -1 ), aromatic ring (1612 and 1441cm -1 ) characteristic functional group; high-resolution mass spectrometry (HRESIMS) gives quasi-molecular ion peak 266.1112 [M+Na] + , it can be determined that the molecular formula of the compound is C 15 h 17 NO 2 , with an unsaturation of 8. combine 1 H and 13 C and HSQCNMR data sh...

Embodiment 3

[0064] This embodiment is a test method for the anti-tobacco mosaic virus activity of 4-acetyl-7-isopropyl-5-methylquinolin-2(1H)-one of the present invention, which is tested by the half-leaf method, specifically:

[0065] The anti-tobacco mosaic virus activity of the compound of the present invention was determined when the mass concentration of the medicament was 20M. On the 5-6 flue-cured tobacco plants, select the leaves suitable for testing (the leaf row is normal, no disease and no insects), and the leaves are evenly sprinkled with fine carborundum, and the spare tobacco mosaic virus source (3.0×10 -3) Spread evenly on the leaves sprinkled with emery. After all the selected leaves are poisoned, immediately place them in a petri dish containing the liquid medicine for 20 minutes, take them out, sprinkle the water drops and liquid on the leaves, and put the two One half of the leaf was restored and placed in an enamel office covered with toilet paper and covered with glas...

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Abstract

The invention belongs to the technical field of tobacco disease prevention and control, and in particular relates to a seed disinfection preparation, its preparation method, use method and application. The disinfection preparation comprises the following components: sodium phosphate, dibenzyl biphenyl polyoxyethylene ether, alcohol solvent and water, and the proportion of each component is: sodium phosphate 8-12wt%, 4-acetyl-7-iso Propyl-5-methylquinoline-2(1H)-ketone 0.05-1wt%, dibenzyl biphenyl polyoxyethylene ether 1-2wt%, alcohol solvent 20wt%, and the balance is water; the 4- The structural formula of acetyl-7-isopropyl-5-methylquinolin-2(1H)-one is as follows: wherein 4-acetyl-7-isopropyl-5-methylquinolin-2(1H)-one is from Newly discovered anti-tobacco mosaic virus alkaloid compounds in gene-edited tobacco stem bark have good anti-tobacco mosaic virus activity. The disinfectant preparation of the invention can effectively remove the tobacco seed-borne mosaic virus and has good application prospects.

Description

technical field [0001] The invention belongs to the technical field of tobacco disease prevention and control, and in particular relates to a seed disinfection preparation, its preparation method, use method and application. Background technique [0002] Tobacco is an important economic crop. Tobacco mosaic disease caused by tobacco mosaic virus (TMV) is an important viral disease in various smoking areas in the world. Tobacco mosaic disease has seriously affected the yield and quality of tobacco leaves. The prominent performance is that the diseased tobacco leaves become smaller, the weight of single leaf decreases, and the number of effective leaves decreases. The general yield loss reaches 5-16%, and when it is serious, it causes extinction. Carrying out research on the prevention and treatment of tobacco mosaic disease is of great significance to improving the yield and quality of tobacco leaves. Seeds carrying viruses are one of the transmission routes of tobacco mosa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/42A01N59/26A01N25/22A01P1/00C07D215/227
CPCA01N43/42A01N59/26A01N25/22C07D215/227
Inventor 刘欣李晶孔维松米其利许永黄海涛朱玲超杨光宇杨叶昆王晋
Owner CHINA TOBACCO YUNNAN IND
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