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Method for inducing in-vitro Chinese yam micro tubers

A technology for micro tubers and yam, applied in the field of agricultural biology, can solve the problems of quality degradation, variety variation, yield reduction and so on

Active Publication Date: 2021-07-06
FUJIAN SANAN SINO SCI PHOTOBIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, yams are mostly propagated and cultivated by cutting off tubers, but long-term use of cutting off tubers is likely to cause quality degradation, reduced yield, and even variety variation, resulting in the abandonment of some local good varieties of yams

Method used

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  • Method for inducing in-vitro Chinese yam micro tubers
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  • Method for inducing in-vitro Chinese yam micro tubers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Inoculate the discarded leaves in the yam tissue culture inoculation into the A1-A6 medium for tuber induction for 75 days. The specific formula of the A1-A6 medium is:

[0027] Basal medium: 3 / 2MS + 3% sucrose + 7g / L agar powder + 0.1% coumarin

[0028] A1: Basal medium+2,4-D 2mg / L+6-BA 0.3mg / L

[0029] A2: Basal medium+6-BA 0.8mg / L+NAA 0.4mg / L

[0030] A3: Basal medium+KT 2mg / L+NAA0.1 mg / L

[0031] A4: Basal medium + 6-BA 2mg / L NAA 0.3mg / L

[0032] A5: Basal medium + 6-BA 1mg / L NAA 0.4mg / L

[0033] A6: Basal medium + 6-BA 0.8mg / L NAA 0.1mg / L

[0034] Experimental results such as Figure 1-6 and as shown in the table below:

[0035]

[0036] It can be seen from this experiment that the combination of 6-BA and NAA is more conducive to the induction of clustered buds and seedlings of yam, and the combination of 2,4-D and 6-BA is beneficial to the induction of bulbs from leaves.

Embodiment 2

[0038] The discarded leaves from the tissue culture inoculation of Chinese yam were inoculated into A7-A12 medium for tuber induction for 75 days. The specific formula of A7-A12 medium was:

[0039] A7: 3 / 2MS+agar powder 7g / L+coumarin 0.1%+sucrose 1%

[0040] A8: 3 / 2MS+agar powder 7g / L+coumarin 0.1%+sucrose 2%

[0041] A9: 3 / 2MS+agar powder 7g / L+coumarin 0.1%+sucrose 3%

[0042] A10: 3 / 2MS+agar powder 7g / L+coumarin 0.1%+sucrose 4%

[0043] A11: 3 / 2MS+agar powder 7g / L+coumarin 0.1%+sucrose 5%

[0044] A12: 3 / 2MS+agar powder 7g / L+coumarin 0.1%+sucrose 6%

[0045] Experimental results such as Figure 7 and as shown in the table below:

[0046]

Embodiment 3

[0048] The discarded leaves from the tissue culture inoculation of Chinese yam were inoculated into A13-A18 medium for tuber induction for 75 days. The specific formula of A13-A18 medium was:

[0049] A13: 3 / 2MS + 3% sucrose + agar powder 0g / L

[0050] A14: 3 / 2MS + 3% sucrose + agar powder 1.5g / L

[0051] A15: 3 / 2MS + 3% sucrose + agar powder 3g / L

[0052] A16: 3 / 2MS + 3% sucrose + agar powder 4g / L

[0053] A17: 3 / 2MS + 3% sucrose + agar powder 5g / L

[0054] A18: 3 / 2MS + 3% sucrose + agar powder 6g / L

[0055]

[0056]

[0057] Comparing the experimental results of comprehensive examples 1-3, it can be seen that adding an appropriate amount of coumarin can promote the direct induction of yam leaves into bulbs, and 3% sucrose is also conducive to bulb induction. As the concentration of sucrose increases, the bulb induction rate declines, but the bulbs Individuals can become larger; the combination of 6-BA and 2,4-D can increase the bulb induction rate.

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Abstract

The invention discloses a method for inducing in-vitro Chinese yam micro tubers. Chinese yam leaves are directly inoculated into a micro tuber induction culture medium for tuber induction. By adopting the method, the leaves can be induced into the micro tubers in one step, the operation is simple and convenient, the propagation coefficient is increased, and the waste leaves of the Chinese yam tissue culture seedlings can be recycled.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a method for inducing isolated yam miniature tubers. Background technique [0002] Chinese yam is a perennial plant of the Dioscoreaceae genus Dioscorea. Ruichang yam is a traditional characteristic vegetable product in Jiujiang City, Jiangxi Province. Its tubers are rich in starch, sugar, protein, vitamins, amino acids and other nutrients, and have strong spleen and kidney benefits. It has the effect of nourishing the lungs and nourishing the lungs. It is a high-quality product that has dual uses of vegetables and medicines. It is known as "Jiangnan ginseng". At present, yams are mostly propagated and cultivated by cutting tubers, but long-term use of cutting tubers is likely to cause quality degradation, yield reduction, and even variety variation, resulting in the abandonment of some local good varieties of yams. Therefore, rational use of yam resources, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李海滨查萍
Owner FUJIAN SANAN SINO SCI PHOTOBIOTECH CO LTD