Glycanase and application thereof in biosynthesis of ligustrazine

A technology of glycanase and ligustrazine, which is applied in the field of glycanase and its application in the biosynthesis of 2,3,5,6-tetramethylpyrazine, can solve the problems of low yield and high cost, and achieve high yield High, reduce preparation cost, low price effect
CN113136377AActive Publication Date: 2021-07-20TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Publication Date
2021-07-20

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Abstract

The invention belongs to the field of biosynthesis, and particularly relates to glycanase and application thereof in biosynthesis of ligustrazine. According to the glycanase, degenerate codons are adopted to replace part of nucleotide in the glyanase, codon optimization is achieved, and the catalytic activity of the glycanase is improved. The invention also provides a preparation method of acetoin, the acetoin is prepared by using microbial cells expressing the glycanase for whole-cell catalysis, and ligustrazine can be further prepared. The method disclosed by the invention is high in substrate conversion rate and high in yield, reduces the cost and is suitable for industrial production.
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Description

technical field

[0001] The invention belongs to the field of biosynthesis, and in particular relates to a glycanase and its application in the biosynthesis of 2,3,5,6-tetramethylpyrazine. Background technique

[0002] 2,3,5,6-tetramethylpyrazine (2,3,5,6-tetramethylpyrazine, TMP), also known as ligustrazine, is a nitrogen-containing heterocyclic compound. It is a colorless crystalline compound with a melting point of 80-82°C, easily soluble in organic solvents such as ethanol and propylene glycol, and slightly soluble in ether. Ligustrazine has a special burnt sweet, nutty and roasted taste, and it exists in nuts, cocoa beans, coffee and other popular foods. In addition, it is also an important component in liquor, which not only helps to enhance the aroma of the liquor, but also has health benefits to a certain extent. Ligustrazine is a recognized safe substance and is a food additive allowed by national standards, with a maximum concentration of 10mg / kg.

[0003] In add...

Claims

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