Synergistic prevention and treatment of beauveria bassiana SB063 and spinetoram on megalurothrips usitatus

A technology of spinosad and Beauveria bassiana, applied in the directions of pesticides, biocides, botanical equipment and methods, etc., can solve the problems of resistance, insignificant drug effect, etc., and achieve good control effects, Environmental pollution-free, the effect of reducing the amount of use

Active Publication Date: 2021-07-23
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the research on the compounding effect of Beauveria bassiana and spinosad, the present invention finds that the compounded insecticides can show a significant synergistic effect, improve the control effe

Method used

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  • Synergistic prevention and treatment of beauveria bassiana SB063 and spinetoram on megalurothrips usitatus
  • Synergistic prevention and treatment of beauveria bassiana SB063 and spinetoram on megalurothrips usitatus
  • Synergistic prevention and treatment of beauveria bassiana SB063 and spinetoram on megalurothrips usitatus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Screening of high virulence strains

[0026] 1.1 Test insects

[0027] Ordinary thrips were harvested from cowpea fields in Zhucun, Zengcheng District, Guangzhou City, Guangdong Province in 2017. After harvesting, they were reared with cowpea pods in RXZ-500C intelligent artificial climate box (Ningbo Jiangnan Instrument Factory) under the temperature (26 ±5)℃, light cycle 12L:12D, relative humidity (70±5)%.

[0028] 1.2 Test strains

[0029] The strains listed for screening belong to Beauveria bassiana, and they are all preserved in the Engineering Research Center of the Ministry of Education for Biological Control, South China Agricultural University, as follows: SP433, SB036, SB062, SB037, SB015, SB063, SB051, SB041 , SB004, SB043, SP016, SB038, SB050, SB006.

[0030] Of these, a deposit of biological material for patent procedures was also carried out for SB063 and SB038. Among them, another patent application will be filed for the content of the inventi...

Embodiment 2

[0052] Example 2 Identification of strain SB063

[0053] (1) Collection of strain SB063

[0054] Strain SB063 was originally isolated in 2010 from the soil under the Halawu tree in the central Helan Mountains and is a native strain in China.

[0055] To collect soil samples, take about 100g of soil 10-15cm below the topsoil, pack them in plastic bags and bring them back to the laboratory for processing. The soil samples were sieved to remove stones and sundries, then 10g of pure soil was suspended in 90mL of 0.1% Tween-80 solution, shaken, and allowed to stand for 15min, and 2mL of the supernatant was diluted in 8mL of 0.05% Tween-80. , formulated as a soil suspension. Inoculate 0.1 mL of the soil suspension on the red Bengal agar medium plate, push the suspension evenly under the surface of the plate with a triangular glass spatula, incubate at 25°C for 3-7 days, and then use an inoculating loop to cut out a single colony and inoculate it on a PDA plate Continue to cultiva...

Embodiment 3

[0077] Embodiment 3 Toxicity determination of different pesticides to common Thrips

[0078] The pesticide film method was used to prepare pesticides with different concentration gradients. A 5cm centrifuge tube (punch holes) was soaked in the liquid for 2 hours, and 1cm of beans (with no holes at both ends) were soaked in the liquid for 10s, dried naturally, and then picked. 20 female adults of common thrips were placed in centrifuge tubes with beans, and 4 replicates of each concentration were reared in an incubator, observed for 2 days, and their mortality was recorded.

[0079]SPSS19.0 software was used to process and analyze experimental data, and one-way analysis of variance was used to analyze the toxicity results of biological / chemical pesticides on thrips, and Tukey's test was used to test the significance of differences to screen high-toxicity pesticides.

[0080] The results showed that the corrected mortality rate of common thrips increased with the increase of the...

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Abstract

The beauveria bassiana SB063 and the spinetoram in an insecticide disclosed by the invention have a remarkable synergistic effect on the poisoning and killing effects of megalurothrips usitatus. Long-term infection biological research and indoor biological assay show that the combined use of beauveria bassiana and spinetoram has a good prevention and treatment effect on megalurothrips usitatus, and the insecticide has a very strong application potential in biological prevention and treatment, can reduce the use amount of chemical pesticides, has the characteristics of low toxicity and low drug resistance, does not pollute the environment, does not have residues, and can favorably delay the occurrence and development of the insecticide resistance of thrip pests.

Description

technical field [0001] The invention belongs to the technical field of biological control. In particular, it relates to the biological control of common thrips, and more specifically, to the synergistic control of common thrips by Beauveria bassiana and spinosad. Background technique [0002] Chemical pesticides play a huge role in pest control, and my country's pesticide usage and output have long ranked first in the world. However, according to statistics, the effective utilization rate of pesticides in the world is less than 30%, and the annual area of ​​chemical pesticides in my country is 280 million hm 2 Above, the application amount reaches 500,000-600,000 tons, and about 80% of the chemical pesticides directly enter the environment (Li Liang, Zhang Zhanying, Sun Huiying, et al. Discussion on pesticide hazards and green plant protection technology [J]. Hubei Plant Protection, 2018, (4): 63-64). With the long-term and large-scale use of chemical pesticides, the "3R"...

Claims

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Application Information

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IPC IPC(8): A01N63/30A01N43/22A01P7/04
CPCA01N63/30A01N43/22
Inventor 吴建辉田庆恒陈月银肖卡特·阿里王兴民邱宝利
Owner SOUTH CHINA AGRI UNIV
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