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Anthocyanin-contained composition and application thereof

A technology of anthocyanin and composition, applied in the field of medical use of anthocyanin, can solve the problems of fruit pulp intolerance to storage, loss of color and biological activity, easy oxidative denaturation, etc., to achieve enhanced anti-oxidation mechanism and enhanced antibacterial effect , strong antioxidant effect

Inactive Publication Date: 2021-07-23
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although mulberry has good health care functions, its pulp is not resistant to preservation, and its main functional component, natural mulberry anthocyanins, is easily oxidatively denatured, losing its proper color and biological activity

Method used

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  • Anthocyanin-contained composition and application thereof
  • Anthocyanin-contained composition and application thereof
  • Anthocyanin-contained composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0034] Embodiment 1 (composition containing grape skin anthocyanins):

[0035] Using choline chloride / glycerol (molar ratio 2:3) deep eutectic solvent as extractant, solid-liquid ratio 1:5, ultrasonic temperature 70 °C, ultrasonic time 60 min to extract grape skin anthocyanins, the prepared Grape skin anthocyanin extract was carried out column separation, using D101 macroporous adsorption resin as adsorbent, eluted with distilled water of 4 times the volume of macroporous resin to remove deep eutectic solvent extractant; with mass concentration as 95% ethanol solution as Desorb the grape skin anthocyanin extract with the eluate, collect the eluate, and evaporate to no liquid at 50°C to obtain the purified grape skin anthocyanin powder; then freeze-dry the purified grape skin anthocyanin powder , passed through a 80-mesh sieve to obtain grape skin anthocyanin powder, and set aside; take 5 g of grape skin anthocyanin powder, mix with 2 g of mannitol-starch (mass ratio 1:1), 0.5 ...

Embodiment approach 2

[0037] Embodiment 2 (composition containing grape skin anthocyanins):

[0038] Choline chloride / ethylene glycol (molar ratio 1:2) deep eutectic solvent was used as the extractant, the ratio of solid to liquid was 1:15, the ultrasonic temperature was 80 °C, and the ultrasonic time was 60 min to extract grape skin anthocyanins. Grape skin anthocyanin extract was carried out column separation, using D101 macroporous adsorption resin as adsorbent, eluted with distilled water of 4 times the volume of macroporous resin to remove deep eutectic solvent extractant; with mass concentration as 95% ethanol solution as Desorb the grape skin anthocyanin extract with the eluate, collect the eluate, and evaporate it to no liquid by rotary evaporation at 50°C to obtain the purified grape skin anthocyanin powder; then freeze-dry the purified grape skin anthocyanin powder, Sieve through 80 mesh to get grape skin anthocyanin powder, set aside; take 6 g of grape skin anthocyanin powder, mix with 4...

Embodiment approach 3

[0039] Embodiment 3 (composition containing grape skin anthocyanins):

[0040] Using choline chloride / lactic acid (molar ratio 1:2) deep eutectic solvent as extractant, solid-liquid ratio 1:8, ultrasonic temperature 60°C, ultrasonic time 100 min to extract grape skin anthocyanins, the prepared grape skin The anthocyanin extract was subjected to column separation, using D101 macroporous adsorption resin as the adsorbent, and eluted with distilled water 4 times the volume of the macroporous resin to remove the deep eutectic solvent extractant; the ethanol solution with a mass concentration of 95% was used as the elution desorb the grape skin anthocyanin extract, collect the eluent, and evaporate to no liquid through 50°C rotary evaporation to obtain the purified grape skin anthocyanin powder; then freeze-dry the purified grape skin anthocyanin powder, and pass Grape skin anthocyanin powder was obtained by 80-mesh sieve, and set aside; take 5 g of grape skin anthocyanin powder, m...

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Abstract

The invention relates to the field of medicinal use of anthocyanin and discloses an anthocyanin-contained composition and application thereof. If anthocyanin is extracted from grape skin, the composition is applied to antioxidation drugs, plays a role in clearing away DPPH free radicals, superoxide anion free radicals (O2<->.) and hydroxyl free radicals (.OH) and has a relatively high reducing property; if the anthocyanin is extracted from mulberries, the composition is applied to bacteriostatic drugs and plays a role in inhibiting staphylococcus aureus, Escherichia coli and bacillus subtilis; and the composition can also be applied to blood sugar reducing, blood fat reducing and atherosclerosis inhibiting and plays a role in inhibiting intestinal alpha-glucosidase, the blood sugar reducing activity of acarbose is enhanced obviously, the synthesis of fats is inhibited, the decomposition of the fats is stimulated, and the composition has blood sugar reducing and blood fat reducing actions and can be used for inhibiting expression of adhesion molecules and inhibiting formation of atherosclerosis.

Description

technical field [0001] The invention relates to the field of medical application of anthocyanin, in particular to a composition containing anthocyanin and its application. Background technique [0002] Free radicals refer to atoms or groups with unpaired electrons formed by the homolysis of covalent bonds in the molecules of compounds under external conditions such as light and heat. Because free radicals contain unpaired electrons, they are extremely unstable (especially hydroxyl radicals), so they will take electrons from neighboring molecules (including fats, proteins, and DNA) to make themselves in a stable state. In this way, the adjacent molecule turns into a new free radical, and then grabs electrons. As a result of such a chain reaction, the structure of the cell is damaged, resulting in loss of cell function, gene mutation, and even death. [0003] Anthocyanins are a class of water-soluble natural pigments that widely exist in plants. They belong to flavonoids and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61K9/14A61P3/10A61P3/06A61P9/10A61K36/605A61K36/87
CPCA61K31/352A61K9/145A61K9/146A61P3/10A61P3/06A61P9/10A61K36/605A61K36/87A61K2236/30Y02A50/30
Inventor 王朝宇张睿毕艳红季青霞杨荣玲丁成新黄龙云
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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