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Application of naphthoquine phosphate in preparation of drug for treating diseases caused by viruses

A technology of naphthoquine phosphate and virus, which is applied in the field of medicine and can solve problems such as no reports on the application of naphthoquine phosphate

Active Publication Date: 2021-07-23
CHENGDU ZIHAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there is no report on the application of naphthoquine phosphate for the preparation of medicines for treating diseases caused by viruses

Method used

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  • Application of naphthoquine phosphate in preparation of drug for treating diseases caused by viruses
  • Application of naphthoquine phosphate in preparation of drug for treating diseases caused by viruses
  • Application of naphthoquine phosphate in preparation of drug for treating diseases caused by viruses

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0019] Experimental Example 1 Study on the Anti-2019-nCoV Activity of Naphthoquine Phosphate in Vitro

[0020] Establish the Vero E6 cell (ATCC-1586) model infected by 2019-nCoV, divide it into naphthoquine phosphate group and chloroquine phosphate group, and use RT-PCR method to determine the effect of drugs on 2019-nCoV by detecting the number of virus replication in the cell supernatant. inhibitory activity (EC 50 ), the results are shown in Table 1.

[0021] Table 1 Inhibitory activity of naphthoquine phosphate on 2019-nCoV virus replication

[0022] drug EC 50

[0023] The results in Table 1 show that on the Vero E6 cell model infected by 2019-nCoV, the half effective concentration EC of chloroquine phosphate to 2019-nCoV 50 The EC50 of naphthoquine phosphate was 0.89 μM, indicating that the inhibitory activity of naphthoquine phosphate on 2019-nCoV was significantly better than that of chloroquine phosphate, which was statistically significant (P<0.05). ...

experiment example 2

[0024] Experimental Example 2 Study on Anti-2019-nCoV Activity of Naphthoquine Phosphate in Vitro II

[0025] 1 Experiment purpose

[0026] To study the inhibitory effect of naphthoquine phosphate on the novel coronavirus (2019-nCoV BetaCoV / Beijing / AMMS01 / 2020) in Vero cells, the CC of naphthoquine phosphate was determined by MTS method (Promega) 50 ; The inhibitory effect of the drug on the new coronavirus (2019-nCoV BetaCoV / Beijing / AMMS01 / 2020) was determined by nucleic acid quantitative method (EC 50 ). And take chloroquine phosphate as a reference.

[0027] 2 Experimental materials

[0028] Naphthoquine phosphate, produced by Sichuan Proud Times Pharmaceutical Co., Ltd., batch number 170401;

[0029] Chloroquine phosphate: product of Sigma Company, lot number BCBJ1498V.

[0030] Vero cells were purchased from the American Standard Biological Collection (ATCC) and preserved by the Virology Laboratory of the Institute of Microbiology and Epidemiology, Academy of Militar...

experiment example 3

[0076] Experimental Example 3 Naphthoquine Phosphate Anti-HCoV-229E Coronavirus Activity Research

[0077] 1 Experiment purpose

[0078] To evaluate the inhibitory effect of naphthoquine phosphate on HCoV-229E coronavirus at the cellular level.

[0079] 2 Experimental materials

[0080] Naphthoquine phosphate (batch number: 170301-1), provided by Sichuan Proud Times Pharmaceutical Co., Ltd., content or purity: 99.0%;

[0081] Ribavirin injection: purchased from Tianjin Jinyao Group Hubei Tianyao Pharmaceutical Co., Ltd., the batch number is 31712252, the specification is 100 mg / ml, diluted to the required concentration when used, and stored in a refrigerator at 4°C.

[0082] Chloroquine phosphate (lot number: BCBJ1498V), Sigma company;

[0083] Passage human liver cancer cells Huh7: preserved by the Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences;

[0084] HCoV-229E was passaged in Huh7.5 cells and stored in a -80°C refrigerator.

[0085] ...

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Abstract

The invention discloses an application of naphthoquine phosphate in preparation of a drug for treating diseases caused by viruses, and belongs to the technical field of drugs. The viruses are coronaviruses and specifically comprise SAS-CoV, MERs-CoV, HCoV-229, HCoV-OC43 and 2019-nCoV.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of naphthoquine phosphate in the preparation of medicines for treating diseases caused by viruses. Background technique [0002] Naphthoquine phosphate was developed by the Institute of Microbiology and Epidemiology of the National Academy of Military Sciences. It is an antimalarial drug after artemisinin drugs. In 1993, Naphthoquine Phosphate Tablets were registered in my country and obtained a Class I New Drug Certificate issued by the Ministry of Health of China. Clinical studies have shown that naphthoquine phosphate has a strong killing effect on various Plasmodium erythrocytic clones, and is the first choice drug for the treatment of falciparum malaria and vivax malaria, and has a good preventive effect. [0003] In terms of systematic classification, coronaviruses belong to the order Nidovirales, the family Coronaviridae, and the genus Coronavirus. Virus...

Claims

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Application Information

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IPC IPC(8): A61K31/4706A61P31/14
CPCA61K31/4706A61P31/14Y02A50/30
Inventor 刘忠荣白红艳叶兵余建红周武春鲍锐
Owner CHENGDU ZIHAO PHARMA
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