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Preparation method of oyster bioactive peptide capable of improving ACE inhibitory activity

A technology for inhibiting activity and active peptides, which is applied in the field of preparation of oyster active peptides, and can solve problems such as differences in product activity

Pending Publication Date: 2021-07-23
DALIAN OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, different amino acids and proteases are added to different reaction substrates, and the reaction temperature, time and other conditions are different, and the activity of the obtained products varies greatly.
So far, there is no report about the preparation of oyster active peptides with enhanced ACE inhibitory activity through the Plastein reaction of exogenously added amino acids and proteases

Method used

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  • Preparation method of oyster bioactive peptide capable of improving ACE inhibitory activity
  • Preparation method of oyster bioactive peptide capable of improving ACE inhibitory activity
  • Preparation method of oyster bioactive peptide capable of improving ACE inhibitory activity

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Embodiment Construction

[0018] A kind of preparation method of the oyster active peptide that can improve ACE inhibitory activity of the present invention is carried out according to the following steps successively:

[0019] a. Shell the oysters and take the meat, soak them in water for desalination until the conductivity drops to less than 180PPM, homogenate, boil, and cool to obtain oyster homogenate;

[0020] b. Add neutral protease with an enzyme activity unit of 300,000 to the oyster homogenate, the amount of enzyme added is 2% of the mass of the oyster homogenate, and the enzymolysis is carried out at pH 7.0 and temperature 50°C for 3 hours to obtain an enzymolysis solution ;

[0021] c. Ultrafilter the enzymatic solution with an ultrafiltration membrane with a molecular weight of 3kDa, retain the permeate, and obtain a small molecule peptide liquid;

[0022] d. The small molecule peptide liquid is subjected to Plastein reaction, the conditions of the Plastein reaction are: the reaction subst...

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Abstract

The invention discloses a preparation method of an oyster bioactive peptide capable of improving ACE inhibitory activity. The preparation method comprises the following steps: performing enzymolysis on oyster homogenate by using neutral protease to obtain oyster small molecule peptide with less than 3kDa of molecular weight, adding quantitative proline and papain by using the oyster small molecule peptide as a reaction substrate, performing Plastein reaction at 60 DEG C for 2 hours, and finally obtaining the oyster bioactive peptide with more than 80% of ACE inhibitory activity through enzyme deactivation, drying and other treatments. The oyster bioactive peptide has a significant hypotensive effect and no side effects on human bodies.

Description

technical field [0001] The invention relates to a preparation method of oyster active peptide, in particular to a preparation method of oyster active peptide which can improve ACE inhibitory activity. Background technique [0002] Oysters are rich in nutrients and contain various active ingredients including ACE (angiotensin-converting enzyme) inhibitory activity. At present, the method of enzymatic hydrolysis of oysters is often used to obtain oyster active peptides, but the obtained active peptides are polypeptide sequences derived from the primary structure of proteins obtained by breaking the peptide bonds of proteins in different places by enzymes. A partial peptide fragment of the amino acid sequence of a raw protein whose activity is restricted. [0003] Plastein reaction, also known as synthetic protein-like reaction or plastic protein reaction, is to add amino acid or / and protease to the reaction substrate (protein hydrolyzate), and catalyze high-concentration prot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06C07K1/34
Inventor 李智博赵前程祁艳霞李莹苏亚文张怡
Owner DALIAN OCEAN UNIV