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A method for the quantitative detection of target proteins in serum by micro-nano optical fiber coupler label-free biosensing

A micro-nano optical fiber, sensing and quantitative technology, applied in the field of biosensing, can solve the problems of high cost and complicated polymerization steps, achieve high sensitivity, and promote the effect of practical application

Active Publication Date: 2022-03-11
广东长光中科生物科技有限公司
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Problems solved by technology

However, the disadvantages of synthesizing such materials are the high cost, complicated polymerization steps, and the convenience and high controllability of synthesizing derivative films remains a challenge.

Method used

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  • A method for the quantitative detection of target proteins in serum by micro-nano optical fiber coupler label-free biosensing
  • A method for the quantitative detection of target proteins in serum by micro-nano optical fiber coupler label-free biosensing
  • A method for the quantitative detection of target proteins in serum by micro-nano optical fiber coupler label-free biosensing

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Embodiment 1

[0038] Embodiment 1: method of the present invention

[0039] (1) Experimental device

[0040] Use a halogen lamp as the light source and focus the light into the OMC using a microscope objective with a numerical aperture of 0.65. Fix the optical microfiber coupler in the fluid cell to deliver the sample solution. A spectrometer with a resolution of 0.3 nm was used as a detector, and the experimental data was obtained through a laptop computer. In order to eliminate the influence of temperature, the biosensing experiment was carried out in a clean room environment, and the temperature was kept at 23 ± 0.2 °C throughout the experiment; the schematic diagram of the experimental device is shown in figure 1 .

[0041] (2) OMC surface functionalization and detection

[0042] 1. Clean OMC with ionized water;

[0043] 2. Immerse OMC in 0.1M KOH solution for 10 minutes, and then wash with ionized water;

[0044] 3. Add 2mg / mL PDDA positive electrolyte solution to soak OMC for 30...

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Abstract

The invention relates to the technical field of biosensing of optical fiber sensors, and discloses a method for quantitatively detecting target proteins in serum by micro-nano optical fiber coupler non-label biosensing. In the invention, the standard serum is pre-adsorbed on the antibody-modified sensor as the antifouling interface, and the linear dynamic detection result under the standard serum environment is used as the calibration straight line. Then it is proposed to use standard serum to reduce the impact of individual differences in human serum on standard-free quantitative detection. Then, using the offset of the standard serum as a reference, after obtaining the deviation between different human sera and the offset of the standard human serum, the target protein in the human serum is quantitatively detected according to the calibration curve. The quantitative detection results of OMC were in good agreement with the clinical detection results. This strategy fills the gap in the standard-free quantitative detection of immunosensors, and further promotes the practical application of micro-nano fiber coupler biosensors.

Description

technical field [0001] The invention relates to the technical field of biosensing of optical fiber sensors, in particular to a method for quantitatively detecting target proteins in serum with a micro-nano optical fiber coupler for standard-free biosensing. Background technique [0002] Optical fiber micro-nanocoupler (OMC) sensors have been widely studied and applied in the field of biosensing due to their low cost, simple structure, low electromagnetic interference, and high sensitivity. However, when detecting target proteins in serum, there is often non-specific adsorption, which increases the background noise signal, hinders detection, and reduces sensor performance. When there is a defect in the functional modification of the immunosensor surface or the antibody does not completely cover the sensor surface, the complex proteins in the serum will be adsorbed to the blank sites on the sensor surface due to hydrophobicity, electrostatic and intermolecular exchange interac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/17G01N21/25G01N33/543
CPCG01N21/17G01N21/25G01N33/54373G01N33/54393
Inventor 吴一辉周文超韦友莲徐阳刘永顺
Owner 广东长光中科生物科技有限公司
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