Biomolecule and inhibitor molecule detection method

Abstract

The invention belongs to the technical field of analysis and detection, and particularly provides a detection method of biomolecules and inhibitor molecules. The biomolecule concentration detection method comprises the following steps that: a to-be-detected substance and hydrogel are mixed to form a mixed solution, the mixed solution is placed one end of an indicating test strip, different amounts of target detection substances cause different hydrolysis degrees of the hydrogel, so that the content of water released by decomposition of the hydrogel is different, different distances of movement can be generated on the pH test strip, and the detection of the target detection object is realized through the length of the moving distance. The problems that a common biomolecule detection method in the prior art is tedious in operation steps, needs to train professionals to operate related equipment, is long in detection time and low in efficiency, causes resource waste and cost increase, and influences the detection sensitivity due to environment background factors such as matrixes exist are solved.

Description

technical field [0001] The disclosure belongs to the technical field of analysis and detection, and specifically provides a detection method for biomolecules and inhibitor molecules. Background technique [0002] The statements herein merely provide background information related to the present disclosure and may not necessarily constitute prior art. [0003] Some biomolecules can be objectively measured as markers and used as evaluation indicators to judge normal biological processes, pathological processes or prognostic responses, and are also important early warning indicators when organisms are damaged or diseased. For this reason, experimenters often need to perform biomolecular tests when investigating pathology or other biological studies. [0004] At present, the commonly used detection methods mainly include enzyme-linked immunoassay, chemiluminescence immunoassay and electrochemical analysis. However, the inventors found that these methods usually have high selec...

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Problems solved by technology

[0005] For the biomolecular detection methods commonly used in the prior art, the operation steps are cumbersome, and professionals need to be trained to operate related equipment, the detection time is long and the efficiency is low, resulting in waste of resources and increased costs. At the same time, there are environmental background factors such as substrates that affect the detection sensitivity. question

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